Unit:
Τομέας Βιολογίας Κυττάρου Και ΒιοφυσικήςLibrary of the School of Science
Author:
Χατζησταματίου Θεοφάνης
Dissertation committee:
Ισιδώρα Παπασιδέρη Καθηγήτρια (Επιβλέπουσα), Λουκάς Μαργαρίτης Ομότ. Καθηγητής, Αικατερίνη Σταυροπούλου-Γκιόκα Ερευνητής Α’, Ελληνική Τράπεζα Ομφαλοπλακουντιακού Αίματος, ΙΙΒΕΑΑ
Original Title:
Απομόνωση , επέκταση και ελεγχόμενη διαφοροποίηση των μεσεγχυματικών βλαστικών κυττάρων του ομφαλοπλακουντιακού αίματος και του ομφαλίου λώρου. Κλινικές εφαρμογές ιστικής ανάπλασης
Translated title:
Isolation, expansion and controlled differentiation of umbilical cord blood and umbilical cord. Mesenchymal stem cells.Clinical applications in tissue regeneration
Summary:
Mesenchymal stem cells (MSCs) are a special class of stem cells: in addition
to their ability of self-renewal and their differentiation capacity, they
exhibit an in vivo immunnomodulatory activity It is therefore considered that
MSCs could contribute to clinical applications of regenerative medicine and in
advanced cellular therapies However, it has been shown that stem cells isolated
from adult tissues, are "aged" and thus show decreased proliferative capacity
and limited functionality.
The aim of this thesis was to determine whether the umbilical cord blood and
umbilical cord tissue, could serve as sources of MSCs for clinical applications.
To achieve that, cells have been isolated from the above sources and cultured.
Their immunophenotype was verified using flow cytometry and their gene
expression using reverse transcription PCR. Finally, their differentiation to
osteocytes, chondrocytes and adipocytes was induced and then verified with
immunocytochemical methods.
From the analysis of the results: 1)It became apparent that MSCs exist in cord
blood but their systematic isolation is impossible with the methods available
at the moment 2)We confirmed the ease of MSCs isolation from umbilical’s cord
Wharton’s. These cells exhibit differences to those of adults, differences,
related to their foetal originand their "young" age, 3)We showed that these
cells could be used as an alternative for tissue engineered constructs, insofar
based on the supply of more “aged” adult MSCs. 4) We, designed and validated an
isolation and cryopreservation protocol of these cells, with a view to their
future use for clinical applications and 5)we showed that culturing foetal
mesenchymal stem cells can achieved without the use of animal sera, using as a
substitute the cord blood serum.
Keywords:
Mesnchymal Stem Cells, Umbilical Cord, Umbilical Cord Blood, Stem cells, Biobanks
Number of references:
258
