Benthic microorganisms as producers of bioactive compounds

Doctoral Dissertation uoadl:1308757 612 Read counter

Unit:
Τομέας Βοτανικής
Library of the School of Science
Deposit date:
2015-09-09
Year:
2015
Author:
Μπουρμπούλη Μαρία
Dissertation committee:
Αμαλία Δ. Καραγκούνη Καθηγήτρια Μικροβιολογίας (Επιβλέπουσα), Βασίλειος Ρούσσης Καθηγητής ΕΚΠΑ, Ευάγγελος Παπαθανασίου Διευθυντής Ερευνών ΕΛ.ΚΕ.Θ.Ε.
Original Title:
Βενθικοί μικροοργανισμοί ως παραγωγείς βιοενεργών ενώσεων
Languages:
Greek
Translated title:
Benthic microorganisms as producers of bioactive compounds
Summary:
The aim of the present PhD thesis was the study of the bacterial diversity
associated with antimicrobial activity of mesophilic heterotrophic bacterial
strains isolated from microbial mats recovered from sediment and chimney
samples from the hydrothermal field of Kolumbo submarine volcano (500 m depth),
in the Aegean Sea. Α qualitative analysis of the heterotrophic mesophilic
bacterial population was conducted and 832 bacterial strains were isolated.
Then discrimination among the isolated strains was performed by using BOX-PCR
method and 230 different profiles were obtained which were tested for
antimicrobial activity against 18 pathogenic and non indicator strains. 89% of
them inhibited growth of at least one target, while 42 strains exhibiting
activity against more than 5 targets, were chosen for sequencing and
phylogenetic analysis. They belonged to the phyla Firmicutes and Proteobacteria
and screened for the presence of polyketide and non ribosomal synthases. 2 (KS)
domains were closely related with hybrid NRPS-PKS and 4 domains with trans-AT
PKS, 4 adenylation domains with antibiotics bacitracin, linear gramicidin and
tyrocydine and 8 domains with lipopetide surfactin. Besides, 3 secondary
metabolites were isolated from a multi-active strain which belong to
2,5-diketopiperazine family.
Keywords:
Κolumbo, Hydrothermal vents, Antimicrobial activity, Mesophiles, Bacteria
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
278
Number of pages:
203
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