Unit:
Τομέας ΙΙ [Οργανική Χημεία – Οργανική Χημική Τεχνολογία – Χημεία Τροφίμων]Library of the School of Science
Dissertation committee:
Ιωάννης Ζαμπετάκης Αναπλ. Καθηγητής ΕΚΠΑ (Επιβλέπων), Κωνσταντίνος Α. Δημόπουλος Καθηγητής ΕΚΠΑ, Ελισάβετ Φραγκοπούλου Επίκ. Καθηγήτρια Χαροκόπειου Πανεπιστημίου ,
Original Title:
Μελέτη και Ταυτοποίηση Δομής των Πολικών Λιποειδών των Ψαριών με Καρδιοπροστατευτικές Ιδιότητες
Translated title:
Study and Structural Elucidation of Fish Polar Lipids with Cardioprotective Properties
Summary:
The purpose of this research is two-fold and concerns initially the
investigation of the existence and structure of biologically active polar
lipids in a solid waste of olive oil industry, namely olive pomace (OP) and
their potential to act as anti-atherogenic compounds. In this project, gilthead
sea bream (Sparus aurata) has been fed with the experimental fish feed enriched
with OP, compounded by adding 8% OP prior to the extrusion. Total lipids of
olive pomace (OP), olive pomace diet (OP diet), fish oil diet (FO diet) and
fish fillets of farmed gilthead sea bream (fish fed with FO diet and OP diet
respectively) were extracted and separated into polar (TPL) and neutral (TNL)
lipids.
The fatty acid composition of TPL of all samples was determined by gas
chromatography (GC-FID). Subsequently, all samples were assessed for their in
vitro activity against washed rabbit platelets aggregation induced by Platelet
Activating Factor (PAF), an active mediator of inflammation who is considered
to be the main cause of atherogenesis and they were further analyzed by gas
chromatography-mass spectrometry (GC-MS) and electrospray-mass spectrometry
(ESI-MS/MS). The high levels of palmitic (16:0), oleic (18:1 cis ω-9), linoleic
(18:2 ω-6) and docosapentanoic acid (DPA 22:5 ω-3) contained in both OP and FO
diets are reflected to the gilthead sea breams fed with the individual diet
respectively, while the gilthead sea bream fed with FO diet displays a decrease
in DPA. All samples contained various glycerophospholipids species. Two PE
species (i.e. PE 18:0/18:0 and PE 18:2/20:4) were identified in OP, OP diet and
fish fed with OP diet and not in FO diet. Our results suggest that these lipids
are likely to be the key polar phospholipids that have the ability to be in
vitro PAF inhibitors, i.e. inhibit and/or regress the formation of
atherosclerotic plaques in blood arteries.
Keywords:
Gilthead sea bream (Sparus aurata), Olive pomace, Cardioprotective properties, Tandem mass spectrometry, Glycerophospholipids
Number of index pages:
161-165
Number of references:
294
