Study of the role of a multipotent transcriptional regulator in the development of the Drosophila melanogaster

Doctoral Dissertation uoadl:1309115 569 Read counter

Unit:
Τομέας Βιοχημείας Μοριακής Βιολογίας
Library of the School of Science
Deposit date:
2014-01-21
Year:
2014
Author:
Γιαννιός Παναγιώτης
Dissertation committee:
Σοφία Τσιτήλου, Αναπλ. Καθηγήτρια ΕΚΠΑ (επιβλέπουσα), Χρήστος Δελιδάκης, Καθηγητής Πανεπιστημίου Κρήτης, Διαμάντης Σίδερης, Αναπλ. Καθηγητής ΕΚΠΑ
Original Title:
Μελέτη του ρόλου ενός πολυδύναμου μεταγραφικού ρυθμιστή στην ανάπτυξη της Drosophila melanogaster
Languages:
Greek
Translated title:
Study of the role of a multipotent transcriptional regulator in the development of the Drosophila melanogaster
Summary:
In the present study, we studied the gene CG12701 (FlyBase) synonym to
vielfaltig) and zld (zelda – Zinc-finger early Drosophila activator) of the
Drosophila melanogaster, that codes for a transcription factor reported as
Zelda. The transcription of the gene zelda was detected in all four
developmental stages of Drosophila by RT-PCR experiments. Moreover, using
specific antibody raised against Zelda, Western Blotting experiments showed
production of the protein throughout development while immunohistochemical
experiments revealed the pattern of production of Zelda in embryonic and larval
tissues. Overexpressing or knocking down Zelda in the developing wing tissue,
showed a certain role of the gene in patterning processes. The phenotypes of
several of these experiments indicated its action within the Notch signaling
pathway. ChIP experiments revealed binding of Zelda to the omb region ~1000 bp
upstream the 5’UTR of the gene. The results of this study show a certain role
of Zelda in wing development and consider it as possible candidate to be
involved in the Notch cascade. However, the results that showed binding of
Zelda on an intronic region of omb cannot exclude the fact of its involvement
in more than one signaling pathways.
Keywords:
Drosophila melanogaster, Transcription factor, Development, Transcriptional regulation, Imaginal wing disc
Index:
Yes
Number of index pages:
7-9
Contains images:
Yes
Number of references:
102
Number of pages:
174
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