Unit:
Τομέας ΙΙ [Οργανική Χημεία – Οργανική Χημική Τεχνολογία – Χημεία Τροφίμων]Library of the School of Science
Author:
Φωτάκης Χαράλαμπος
Dissertation committee:
Λιούνη Μαρία Αναπληρώτρια Καθηγήτρια ΕΚΠΑ (επιβλέπουσα), Λυμπεροπούλου Καραλιώτα Αλεξάνδρα Αναπληρώτρια Καθηγήτρια ΕΚΠΑ, Ζερβού Μαρία Ερευνήτρια Γ’ ΕΙΕ
Original Title:
Μεταβολική μελέτη και σύγκριση αποσταγμάτων με φυσικοχημικές και αναλυτικές μεθόδους
Translated title:
A metabolic study through the comparison of grape marc distillates with physicochemical and analytical methods
Summary:
The endeavor of our laboratory in deciphering the NMR metabolic fingerprint of
Greek grape marc distillates (tsipouro and tsikoudia) is presented. In this
context, 180 samples of indigenous and international varieties, stemming from
major vine growing regions of Greece were investigated. The monitoring protocol
addressed the global metabolic profile of untreated samples and accomplished
the unambiguous assignment of 53 metabolites. NMR spectra were acquired by
applying the robust, sensitive and rapid WET1D NMR pulse sequence, which
succeeded to unveil the presence of minor compounds in a high ethanol matrix.
PCA classified the samples according to their provenance, incorporating also
information related to the variety, vintage year, aging and production
processes within each formed regional assembly. Metabolites such as fusel
alcohols, polyols, ethyl esters, mono- and di-saccharides were associated with
the classification of samples. OPLS-DA and O2PLS-DA related metabolites to the
provenance and genotype of the samples and also probed to the potential
influence of the vintage effect. Additionally, the transference of compounds
from the cask to the beverage during the aging procedure was framed. Furans and
phenolics were identified in the aged distillate stemming from the oak barrel.
In this context, the antioxidant activity of aged grape marc distillates was
evaluated with the implementation of six spectrophotometric assays. Finally,
metabolite profiling underlined the influence of the fermentation and
distillation procedures.
Keywords:
Grape marc distillate, Multivariate data analysis, NMR, Metabolic profiling, Antioxidant activity
Number of index pages:
8-12
Number of references:
104
File:
File access is restricted.
document.pdf
6 MB
File access is restricted.
