Unit:
Τομέας Βιολογίας Κυττάρου Και ΒιοφυσικήςLibrary of the School of Science
Author:
Τζουνάκας Βασίλειος
Dissertation committee:
Ισιδώρα Σ. Παπασιδέρη Καθηγήτρια (Επιβλέπουσα), Βασιλική Αλεπόρου-Μαρίνου Αναπληρώτρια Καθηγήτρια, Ιωάννης Π. Τρουγκάκος Επίκουρος Καθηγητής
Original Title:
Σηματοδότηση κυτταρικής γήρανσης και θανάτου στα ερυθρά αιμοσφαίρια in vivo και in vitro
Translated title:
Red bloοd cell aging and death signaling in vivo and in vitro
Summary:
Like every cell removed from its natural environment, red blood cells (RBC)
stored in blood banks undergo a series of physiologic alterations that
collectively called "Storage Lesion”. These ex vivo alterations refer to
biochemical, mechanical and membrane changes. The scope of this thesis was to
clarify how the RBC aging and death signaling proceeds during the storage
period in various conditions (such as leukoreduction) used in transfusion
practice, as well as the possible relevance of these signaling events with
intrinsic physiological characteristics of eligible blood donors in vivo. For
that purpose, venous blood (N=215) and ex vivo stored RBC (N=26 blood units)
from healthy male donors who met blood donation criteria were used. Samples
were tested for a variety of hematological, biochemical and biological
parameters. According to the results, leukoreduction appears to improve several
storage lesion parameters. Moreover, the variation observed in
hematologic/biological characteristics in vivo (like uric acid or fetal
hemoglobin) is associated with the erythrocytes’ susceptibility to storage
lesion. To summarize, the quality of stored RBC is not only related to storage
duration. Other factors such as the processing of the transfusion units and
donor’s inherent characteristics may play a greater role than the ex vivo
preservation time.
Keywords:
Red blood cells, Transfusion, Storage lesion, Leukoreduction, Donor variation
Number of references:
359
