Unit:
Τομέας ΦΑΡΜΑΚΕΥΤΙΚΗΣ ΤΕΧΝΟΛΟΓΙΑΣLibrary of the School of Science
Dissertation committee:
Α.Λ. Σκαλτσούνης Καθηγητής (Επιβλέπων), Σ. Μητάκου Καθηγήτρια, Ν.Αλιγιάννης Επίκ. Καθηγητής
Original Title:
Implementation of green extraction and isolation methodologies for the recovery of bioactive compounds from olive-growing products and by-products
Translated title:
Εφαρμογή πράσινων μεθοδολογιών εκχύλισης και απομόνωσης για την παραλαβή βιοδραστικών ενώσεων από προϊόντα και παραπροϊόντα της ελαιοκομίας
Summary:
The experimental work of this PhD thesis has been scheduled on a three-axis
base. The aims that were set, they concerned the optimal exploitation of four
different by-products of
the table olive, olive oil and olive kernel oil industries, for the recovery of
specific high added-value compounds with well-known biological properties.
Those natural products were desired either in the form of enriched fractions or
pure compounds. The prerequisite was to utilize as eco-friendly technologies as
possible for extraction and purification and to optimize the processes in
laboratory as well as in pilot scale. Thus, trustworthy estimation of
industrial feasibility is provided. The main technologies that were implicated
for the recovery of the target compounds are:
Supercritical fluid extraction
Pressurized liquid (and subcritical water) extraction
Liquid/liquid extraction Adsorption resin technology
Centrifugal Partition Chromatography The by-products that were the subject of
the tracing/ extraction/ enrichment/ identification/ quantification on studies
for this PhD thesis were the following:
Olive leaves (by-products of the pruning of the trees and of the olive fruit
harvest)
Olive mill pomace waste (biphasic process)
Table olive process wastewater (by-product of the debittering process for the
production of table olives)
Olive kernel oil deodorization distillates (by-product of the refining
process for the production of olive kernel oil from olive pomace)
The natural compounds that were targeted for their recovery, enrichment and
isolation due to their well-established (and still under research) biological
properties and their application in pharmaceutical, phytotherapeutical,
nutraceutical, cosmetic and cosmeceutical industries were respectively:
Oleuropein
Hydroxytyrosol
Squalene
In all cases, not only biomarkers’ content but also the overall chemical
profile of the extracts was studied. The applied scientific interest of this
work was that novel green
extraction technologies and combinatorial processes as well as experimental
design were implemented in lab and pilot scale for the exploitation of certain
by-products that occur in very large quantities every year as a result of the
agricultural/ industrial processing of the olive tree, with the aim of
recovering high added value natural products.
Keywords:
Olive growing by-products, Green extraction technologies, Supercritical carbon dioxide, Countercurrent chromatography, Design of experiments
Number of references:
116
