Unit:
Τομέας ΒοτανικήςLibrary of the School of Science
Dissertation committee:
Γιώργος Διαλλινάς Καθηγητής (Επιβλέπων), Σταύρος Χαμόδρακας Ομότ, Καθηγητής, Ευστάθιος Φριλίγγος Καθηγητής
Original Title:
Structure-function and evolutionary relationships in purine/pyrimidine transporters of the NCS1 and NCS2 families
Translated title:
Σχέσεις δομής-λειτουργίας και εξελικτικές σχέσεις μεταφορέων πουρινών και πυριμιδινών των οικογενειών NCS1 και NCS2
Summary:
The NCS1 and NCS2/NAT families include proteins characterized as H+/Na+
symporters specific for the uptake of nutrients, such as purines, pyrimidines,
vitamins
and related metabolites. The NCS1 family includes members in bacteria, fungi and
plants, whereas NCS2/NAT members are present in almost all domains of life. The
current study concerns the structural and functional analysis of proteins
belonging in
NCS1 and NCS2 families in the fungus Aspergillus nidulans, a model-organism
widely used in studies related to transporters and cellular mechanisms.
This study is divided in four distinct chapters: (i) functional and structural
analysis of the major purine transporter AzgA, (ii) functional characterization
of
Aspergillus brasiliensis NCS2/NAT proteins expressed in A. nidulans, (iii)
functional
and structural analysis of the purine/cytosine transporter FcyB, (iv)
functional and
structural analysis of the uracil transporter FurD and investigation of
“orphan” Furs
function.
Keywords:
Transport proteins, Purines/pyrimidines, Structure-function, Evolution, Aspergillus nidulans
Number of references:
162
