Διατμηματικό / Διϊδρυτικό ΠΜΣ Μοριακή Ιατρική
Library of the School of Health Sciences

2012-10-04

2012

Τρίμπαλης Φίλιππος

Καθ. Νικόλαος Ανάγνου, Ερευνήτρια Α΄ Βλάχου, Ερευνήτρια Γ΄ Σοφία Κοσσίδα

Ανίχνευση πρωτεϊνικών αλληλεπιδράσεων σε ολόκληρα προκαρυωτικά και ευκαρυωτικά γονιδιώματα με χρήση μεθόδων ανάλυσης συγχωνευμένων πρωτεϊνικών δομικών ενοτήτων.

Greek

In this work, the Domain Fusion Analysis (Rosetta Stone method) was applied,
including 19 organisms throughout the branches of the tree of life, against
Trypanosoma brucei, a protozoan, which causes African Trypanosomiasis or
sleeping sickness in humans, which in some cases it is lethal, while the most
modern available vaccines have severe side-effects. The purpose of this
analysis was to detect protein-protein interactions which in turn could be
pharmacologically studied, and the development of drugs against this disease to
block the specific interaction could follow. Overall, 49 possible
protein-protein interactions were detected, and there was an effort to evaluate
even the ones including hypothetical proteins, which led to a suggestion of
possible molecular function or involvement in a certain biological process.
This work has produced valuable results which could be further studied through
structural biology or other experimental approaches so as to validate those
protein-protein interactions proposed here. Also, the significance of this
technique was appreciated by comparing it with other methods in this field for
such events. To conclude with, an evolutionary analysis of the proteins
involved was carried out, showing that, events as gene fusion or gene fission
can happen to all organisms with other events being more common and others not
so often. Some multiple events were also detected that seem to be highly
complex, seeking to be evolutionary explained one by one.

Gene fusion events, Trypanosoma brucei, Protein-protein interactions, Rosetta Stone method, Comparative genomics

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https://pergamos.lib.uoa.gr/uoa/dl/object/1310064