Διατμηματικό / Διϊδρυτικό ΠΜΣ Μοριακή Ιατρική
Library of the School of Health Sciences

2016-05-18

2016

Θάνου Ειρήνη

Στυλιανοπούλου Φ. Καθηγήτρια, Τμήμα Νοσηλευτικής, ΕΚΠΑ

Απόκριση μη-νευρογενετικων περιοχών στον μηχανικό και χημικό τραυματισμό της υποκοιλιακής ζώνης ενήλικων μυών.

Greek

Ιn the adult mammalian brain neural
stem/progenitor cells (NPCs) have a spatially restricted distribution, and
neurogenesis takes place only in specific brain areas. Thus, all brain regions
beyond
the SVZ and dentate gyrus are considered “non-neurogenic”. Recently, it is
proposed
that a low level of neurogenesis occurs also in “non-neurogenic regions”, such
as the
striatum and, to a lesser extent, the cortex. Importantly, brain lesions have
succeeded to unmask neurogenic activity in such regions in laboratory rodents.
In
this study we aim to investigate whether disturbance of the adult mouse SVZ,
activates cells of neighboring non-neurogenic regions to acquire a neural
progenitor
potential. To this end, we have performed a protocol aiming to reduce the
SVZ-stem
cell population, using a combination of mechanical trauma in the SVZ area, to
activate quiescent or slowly proliferating NPCs, followed by subsequent
repeated,
stereotaxic intraventricular injections of the mito-toxic agent arabinoside-C
(Ara-C),
that kills rapidly proliferating NPCs.
Finally we sacrifice the animals s 6 weeks after the primary trauma.
The results of this study support the hypothesis that the injection of
mitotoxic AraC in the lateral ventricles is responsible for the the disruption
of the
SVZ and the induction of the ectopic presence of newborn neurons in
nonneurogenic
areas.The
fact that several mitotoxic factors are widely used as chemotherapeutic agents
and
that patients undergoing chemotherapy exhibit progressive cognitive deficits,
raises
the question whether these treatments lead to a disruption of neurogenesis.
Therefore, further study of this SVZ-disorganization model will shed light into
important questions related to NPCs and neighboring parenchymal brain cells’
response to chemotherapeutic treatment.

Adult Neurogenesis, Non-neurogenic regions, Striatum, Trauma, Subventricular zone

No

0

Yes

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https://pergamos.lib.uoa.gr/uoa/dl/object/1310250