Αναπλ. Καθηγήτρια Κονδή-Παφίτη Αγάθη (επιβλέπουσα) , Αναπλ. Καθηγήτρια Καΐρη-Βασιλάτου Παρασκευή , Καθηγητής Γρηγορίου Οδυσσέας Καθηγητής
The purpose of this study is to record the distribution and changes of relaxin
receptor (LGR7) and collagen of fetal membranes in placentas from normal and
abnormal pregnancies (preterm premature rupture of membranes, fetal chromosomal
abnormalities, intrauterine growth retardation of the fetus (IUGR), pregnancies
complicated with diabetes mellitus).
MATERIALS-METHODS: 56 samples of placental tissue were examined: a) 16 normal
placentas at term pregnancy, b) 11 second-trimester placentas after preterm
premature rupture of membranes (gestational age 15-26 weeks), c) 10
second-trimester placentas after termination of pregnancy due to chromosomal
abnormality of the fetus (gestational age 14-26 weeks), d) 11 placentas from
pregnancies with intrauterine growth retardation of the fetus (IUGR) and e) 8
placentas from pregnancies complicated with diabetes mellitus (DM). Cases with
remarkable alterations of chorioamnionitis were excluded. All samples are
between second trimester of pregnancy and after, when levels of plasma relaxin
are constant. All specimens were examined by routine method, multiple
histological sections were stained with hematoxylin / eosin and
immunohistochemical method was used for the study of the relaxin receptors LGR7
and collagen type I and III. The immunoreactivity for LGR7 was recorded in the
following tissues: amniotic epithelium cells, decidual cells, trophoblastic
elements (cytotrophoblast, syncytiotrophoblast and intermediate trophoblast)
fibroblasts and blood vessels of the decidual matrix. The entire evaluation was
based on semiquantitative method: Depending on the extent of immunostaining,
the samples were evaluated with positive (+) when they had positive
immunoreactivity in 25% of the cells, (+ +) when they had positive
immunoreactivity in 50% of the cells and as (+ + + ) when over 50% of the cells
had positive immunoreactivity.
We also evaluated the intensity of immunostaining.
1) LGR7 relaxin receptors expressed strongly in fetal membranes, with a
particular distribution in the decidual cells, the amniotic epithelial cells,
the syncytiotrophoblast, the blood vessels and fibroblasts of the matrix.
2) In the cases of preterm premature rupture of membranes, there was increased
relaxin receptor expression in fetal membranes, indicating a specific role of
this hormone in this particular pathology of pregnancy. This increase was
observed particularly on the decidual cells, the amniotic epithelial cells and
the intermediate trophoblast, and accompanied by a decrease in collagen type
3) In the cases of intrauterine growth retardation observed a decrease in the
expression of relaxin receptors in membranes, particularly in amnion and
4) In pregnancies complicated with diabetes mellitus there was increased
relaxin receptor expression in fetal membranes, particularly in the amnion, the
decidua, the syncytiotrophoblast, and the intermediate trophoblast.
5) There were no significant differences (not statistically significant, p>
0,05) in the expression of LGR7 receptors and the distribution of collagen
between cases of mature placentas before and after labor.
relaxin receptor (LGR7), collagen, decidua, placenta, pregnancy