Μελέτη έκφρασης παραγόντων του μονοπατιού απόκρισης σε βλάβες του DNA σε προκαρκινικές και καρκινικές αλλοιώσεις κεφαλής και τραχήλου του ανθρώπου

Postgraduate Thesis uoadl:1312417 247 Read counter

Unit:
Διατμηματικό / Διϊδρυτικό ΠΜΣ Μοριακή Ιατρική
Library of the School of Health Sciences
Deposit date:
2012-10-03
Year:
2012
Author:
Γλυτσού Χριστίνα
Supervisors info:
Επίκουρος Καθηγητής Αθανάσιος Κοτσίνας (επιβλέπων), Καθηγητής Βασίλειος Γοργούλης, Ερευνητής Β' Δημήτριος Κοντογιάννης
Original Title:
Μελέτη έκφρασης παραγόντων του μονοπατιού απόκρισης σε βλάβες του DNA σε προκαρκινικές και καρκινικές αλλοιώσεις κεφαλής και τραχήλου του ανθρώπου
Languages:
Greek
Summary:
Introduction: DNA damage response (DDR) emerges as a cellular tumorigenesis
barrier. The issue of how early during carcinogenesis deregulation of DDR
factors occurs, releasing the cell from the suppressive effects of the DNA
damage checkpoint pathway, remains unresolved.
Aim: Study of the expression of DDR factors in human precancerous and cancerous
lesions in laryngeal epithelium.
Methods: Paraffin-embedded material from 17 patients with neoplasmatic
laryngeal lesions was examined. All cases included carcinoma, as well as
normal, hyperplastic and/or dysplastic counterparts. Immunohistochemistry
against the DDR markers γΗ2ΑΧ-Ser139 and pChk2-T68, the tumor-suppressor TP53
and p21WAF1/CIP1 was performed.
Results-Conclusions: Activation of the DDR was observed in precursor lesions
and laryngeal carcinoma, but not in normal tissue. The result is consistent
with previous studies in human lung, bladder, breast and colon tumors.
Immunohistochemical staining against the effector, TP53, and its target,
p21WAF1/CIP1, showed high expression levels of the first, but on the other hand
very low expression levels of the latter in dysplasia and carcinoma. Therefore,
it can be assumed that TP53 is mutated in high grade precancerous and cancerous
lesions, so as the protein product is not capable of inducing cellular
senescence – through the induction of p21WAF1/CIP1 transcription – or
apoptosis, resulting in the loss of its tumor suppressive role.
Keywords:
Carcinogenesis, Larynx, DNA damage response, Precancerous lesions, TP53
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
134
Number of pages:
104
File:
File access is restricted.

document.pdf
3 MB
File access is restricted.