Study of the role of Cytokeratin 14 expressing cellular subpopulation in the physiology of the mouse urothemium

Postgraduate Thesis uoadl:1312550 245 Read counter

Unit:
Διατμηματικό / Διϊδρυτικό ΠΜΣ Μοριακή Ιατρική
Library of the School of Health Sciences
Deposit date:
2014-12-19
Year:
2014
Author:
Παρασκευοπούλου Βαρβάρα
Supervisors info:
Αν. Καθ. Ιπποκράτης Κιάρης, Ερευνητής Γ' Απόστολος Κλινάκης, Ερευνήτρια Γ΄Λασκαρώ Ζαγοραίου
Original Title:
Μελέτη του ρόλου του κυτταρικού υποπληθυσμού που εκφράζει την κερατίνη 14 στη φυσιολογία του ουροθηλίου του ποντικού
Languages:
Greek
Translated title:
Study of the role of Cytokeratin 14 expressing cellular subpopulation in the physiology of the mouse urothemium
Summary:
The urothelium is a multi-layered epithelium that extends from the renal pelvis
to the proximal urethra. It consists of three cell layers: the superficial, the
intermediate and the basal cell layer. It represents a quiescent epithelium,
which is regenerated promptly, following acute lesions. The aim of this study
was to investigate the role of keratin 14 expressing basal cells of the
urothelium in tissue homeostasis. For this purpose, we performed a
developmental study, which revealed that keratin 14 expressing cells are
distinguishable in mouse embryonic urothelium on Ε16.5. Furthermore, C57bl6
adult mice were treated with cyclophosphamide, in order to induce a quick cycle
of injury and regeneration. KRT14+ cells increased in number six hours after
injury, while ten days after treatment their levels tend to be equivalent to
those of the control group. Finally, we created two transgenic mice expressing
Cre recombinase under the control of the keratin 14 promoter, for lineage
tracing experiments. We conclude that KRT14+ cells have the ability to generate
differentiated cells both in the intermediate and in the superficial cell
layer. This observation could be an indication that KRT14+ cells constitute a
possible progenitor cell pool within the basal layer in the urothelium.
Keywords:
Urothelium, Cyclophosphamide, Keratin 14, Bladder progenitors, Transgenic mice
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
117
Number of pages:
84
File:
File access is restricted only to the intranet of UoA.

document.pdf
3 MB
File access is restricted only to the intranet of UoA.