Μελέτη του ρόλου του πολλαπλασιασμού Τ λεμφοκυττάρων στην ανάπτυξη Πειραματικής Αυτοάνοσης Εγκεφαλομυελίτιδας

Postgraduate Thesis uoadl:1312553 255 Read counter

Unit:
Διατμηματικό / Διϊδρυτικό ΠΜΣ Μοριακή Ιατρική
Library of the School of Health Sciences
Deposit date:
2016-01-27
Year:
2016
Author:
Ξενιάς Παντελεήμων
Supervisors info:
Μανουσάκης Μενέλαος, Καθηγητής, Probert Lesley, Τζιούφας Αθανάσιος, Καθηγητής
Original Title:
Μελέτη του ρόλου του πολλαπλασιασμού Τ λεμφοκυττάρων στην ανάπτυξη Πειραματικής Αυτοάνοσης Εγκεφαλομυελίτιδας
Languages:
Greek
Summary:
In 2014, the project of our research team revealed that intradermal injection
of myelin epitopes, which have been chemically conjugated to mannan, can lead
to peripheral T cell tolerance and prevent the onset of Experimental Autoimmune
Encephalomyelitis (EAE) in mice. EAE represents an animal model for multiple
sclerosis. In addition, in vitro studies have linked tolerance to lower
antigen-specific proliferation and the development of peptide-specific T cell
anergy, despite the fact that the expression of Th1 and Th17 effector cytokines
was not impaired1. The objective of this Thesis was to examine in vivo whether
the protective effects of mannan-cojugated peptides are mediated by changes in
proliferation or trafficking of activated T cells into the CNS during EAE.
Chimeric mice were generated, in which MOG35-55(MOG)-specific T cells isolated
from 2D2 MOG-specific T cell receptor transgenic mice2 that had previously been
vaccinated with mannan-MOG or PBS control, were labeled with fluorescent CFSE
and adoptively transferred into congenic (CD45.1) recipients with ongoing
MOG-EAE. Our experiments indicate that mannan-conjugated peptide did not affect
the migration of activated MOG-specific T cells into the CNS parenchyma
compared to PBS-treated control cells, although it did have an impact on their
proliferation responses.
Keywords:
Experimental Autoimmune Encephalomyelitis (EAE), Multiple sclerosis, T cells, Anergy, CNS
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
101
Number of pages:
VII, 55
File:
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