Unit:
Κατεύθυνση Αναπαραγωγική-Αναγεννητική ΙατρικήLibrary of the School of Health Sciences
Author:
Μαρινόπουλος Σπυρίδων
Supervisors info:
Λουτράδης Δημήτριος, Δρακάκης Πέτρος,Καλλιανίδης Κων/νος
Original Title:
Πολυμορφισμός Ser680asn του γονιδίου του υποδοχέα της FSH(FSHR) και εξωγενώς χορηγούμενη δόση FSH σε πρωτόκολλα IVF/ICSI
Summary:
Aim: Previous studies concluded that a multigenic model including specific FSHR
genotype patterns may explain response to FSH. Our aim was interrelation of
follicle-stimulating hormone receptor Ser680Asn polymorphism with exogenous
administered FSH during IVF/ICSI protocols.
Material and method: Patients were divided in 3 groups according to total dose
of exogenous administered FSH. In this retrospective study, 166 women were
included, all presenting to Human Reproduction – IVF Unit of the 1st Ob/Gyn
Department of Athens University Medical School, in “Alexandra” Hospital. All
women included experienced infertility problems for at least one year.
Inclusion and exclusion criteria were specific. IVF protocols are described
meticulously. Clinical, biochemical and genetic parameters were recorded. FSH
receptor polymorphism detection was conducted by peripheral blood sampling,
photometry, real-time polymerase chain reaction and melt curve genotyping.
Results: Genotype combinations of FSH receptor Ser680Asn gene in IVF and ICSI
patients were: Ser/Ser 19.9% (33/166), Ser/Asn 51.8% (86/166) και Asn/Asn 28.3%
(47/166). Women with Ser/Ser polymorphism received total exogenous FSH dose
2500 – 5000 IU at higher rate (78.8%) than women with other polymorphisms
(50.0% for Ser/Asn and 55.3% for Asn/Asn). Patients with Ser/Ser succeeded in
becoming pregnant in 4.2%, those with Ser/Asn in 23.3% and those with Asn/Asn
in 23.3%
Conclusions: Homozygous women for FSHr Ser/Ser polymorphism required higher
exogenous doses of FSH. Pregnancy rates do not differ according to the
polymorphisms studied.
Keywords:
Polymorphism, SER680ASN, Gene, Receptor, IVF/ICSI
Number of index pages:
114
Number of references:
189
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