Διατμηματικό / Διϊδρυτικό ΠΜΣ Μοριακή Ιατρική
Library of the School of Health Sciences

2012-07-03

2012

Καπαρέλου Μαρία

Καθηγητής Μ.Κουτσιλιέρης, Αν. Καθηγητής Χ.Κόνσολας, Λέκτορας Α. Αρμακόλας

In vitro παραγωγή πεπτιδίου Ε (ενεργό μέρος IGF1-Ec) σε E.coli

Greek

The aim of this study is in-vitro production of peptide E (active part of
IGF-Ec) in E.Coli, aspire to the production of peptide E of IGF1-Ec and to the
determination of biological ‘route’ that is connected with the action of IGF-Ec
after pT7-FLAG-MAT-Tag 2 expression vector it assigns in peptide E, which is
cloned in this, metal affinity properties. Thus the proteins of expression
vector might be withheld from [Ni]+ columns, before and after interaction with
intracellular and membrane proteins.
Initially composition of DNA took place that leads to the production of peptide
E of IGF-1Ec. From muscular tissue that has been proved that it expresses
IGF-Ec we isolated RNA and then RT –PCR was taken place with selected primers.
The RT-PCR product was then inserted in the expression vector and then it was
cloned in the expression strain of E.coli BL21. The BL21 strain allows the
expression of the peptide that was inserted in the expression vector. The
induction of expression was successful and with Coomassie and Western Blot we
confirmed the insertion of the peptide in E.coli.

Bacteria , Cloning, Metal ions, Protein induction, Plasmid

No

0

Yes

75

83

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https://pergamos.lib.uoa.gr/uoa/dl/object/1314903