Unit:
Κατεύθυνση Στοματολογία (Κλινικές Ειδικεύσεις)Βιβλιοθήκη Οδοντιατρικής
Author:
Γκίλας Χαράλαμπος
Supervisors info:
Αλεξάνδρα Σκλαβούνου, Καθηγήτρια, ?ιευθύντρια του Εργαστηρίου της Στοματολογίας, Ευανθία Χρυσομάλη, Επίκουρη Καθηγήτρια Στοματολογίας, Κωνσταντίνος Τόσιος, Επίκουρος Καθηγητής Στοματολογίας
Original Title:
Ανοσοϊστοχημική μελέτη της έκφρασης των πρωτεϊνών Pax9 και Bmp4 σε κεντρικά και περιφερικά γιγαντοκυτταρικά κοκκιώματα των γνάθων
Translated title:
Immunohistochemical evaluation of Pax9 and Bmp4 expression in central and peripheral giant cell granulomas of the jaws
Summary:
Central Giant Cell Granuloma (CGCG) is a benign intraosseous lesion of the jaws
characterized histologically by an abundance of multi-nuclear giant cells
(MNCs) in a stroma of spindle shaped mononuclear cells (MNs). Peripheral Giant
Cell Granuloma (PGCG) is a lesion of the gingiva considered by some researchers
as the extraosseous analogue of CGCG. Giant Cell Tumor (GCT) appears in the
long bones and has a similar histologic appearance but a more aggressive
biological behavior compared to CGCG.
It has been shown in previous research that CGCGs and PCGCs express the
protein Msx1, a known regulator of odontogenesis and an ectomesenchymal marker.
Msx1 highly interacts with Pax9 and they both regulate the expression of Bmp4.
The aim of this study was to evaluate the expression of the two latter by
immunohistochemistry and correlate with the pathogenesis of giant cell lesions.
The intensity and percentage of stained cells was evaluated and the results
compared statistically.
MNs and MNCs type I of CGCGs were Pax9 and Bmp4 positive. The same cell
populations were Pax9 negative and Bmp4 weakly positive in PGCGs.
The expression of Pax9 and Bmp4 by the mononuclear component of CGCGs is an
indication of the ectomesenchymal origin of those cells. These findings may
provide an explanation for their exclusive appearance on the edentulous areas
of the jaws in younger ages and maybe their different biological behavior from
GCTs. Different expression pattern from PGCGs probably shows different
pathogenesis.
Keywords:
Pax9, Bmp4, odontogenesis, ectomesenchyme, giant cell lesions
Number of references:
250
