Summary:
Aim: The aim of this study was to examine in vitro the effect of remnants of
the two most common intracanal medicaments used in regenerative endodontics,
triple antibiotic paste (TAP) and calcium hydroxide (CH), in the survival of
stem cells from exfoliated or deciduous teeth.
Materials and methods: Single rooted teeth were used to form three groups of
six specimens each. After cleaning and shaping, in the first group no
medicament was used, whereas in the second and third group TAP and CH were
applied respectively. A week later medicaments were removed and 105 cells were
placed on every specimen. All roots were then stored in cell culture medium for
seven days. Afterwards all specimens were removed and were examined under a
confocal microscope to determine the number of attached cells to the root
surface and to evaluate cell morphology.
Results-Conclusions: Cell numbers of second and third group were found
significantly greater than those of the first group. No differences were found
between second and third group. Also cells that grew on roots treated with CH
were found to be flattened with well formed nuclei in their majority, in
opposition with the other two groups, characteristics that make them more able
to survive, multiply and differentiate.
Keywords:
Stem cells, Regenerative endodontics, Calcium hydroxide, Triple antibiotic paste
