Unit:
Κατεύθυνση Ορθοδοντική (Κλινικές Ειδικεύσεις)Βιβλιοθήκη Οδοντιατρικής
Author:
Καραμεσίνης Κωνσταντίνος
Supervisors info:
Επίκουρος Καθηγητής κ. Ηλίας Μπιτσάνης, Επίκουρη Καθηγήτρια κ. Ελένη Βασταρδή, Αναπληρώτρια Καθηγήτρια κ. Ευθυμία Μπάσδρα
Original Title:
Effect of continuous hydrostatic pressure on chondrocyte differentiation
Translated title:
Η επίδραση της συνεχούς υδροστατικής πίεσης στη διαφοροποίηση των χονδροκυττάρων
Summary:
Background/Objective: Mechanical forces influence differentiation and
maturation of chondrocytes in vitro. However, the series of biochemical events
following continuous force application to chondrocytes remain largely
unexplored. The aim of the present study was to investigate the long-term
effects of hydrostatic pressure (HP) on chondrocyte differentiation, as
indicated by protein levels of the chondrocyte differentiation markers SOX9 and
RUNX2 transcription factors, as well as on the transcriptional activity of
SOX9, as determined by pSOX9 levels.
Materials and Methods: ATDC5 cells were exposed to 14.7kPa of continuous HP for
12, 24, 48 and 96 hours at the presence of maintenance medium or
insulin-supplemented differentiation medium (ITS). ATDC5 cells cultured in ITS
medium for the same time-points served as controls. Cell extracts for each
time-point and experimental condition were assessed for SOX9, pSOX9 and RUNX2
protein levels using Western immunoblotting analysis.
Results: Exposure of ADTC5 cells to HP resulted in an early drop in SOX9 and
pSOX9 protein levels at 12 hours followed by an increase at 24 hours onwards. A
reverse pattern relatively to SOX9 was observed for RUNX2 protein, which
reached peak levels at 24 hours of HP treatment of chondrocytes in ITS culture.
Twelve and 24 hours of HP treatment were revealed as critical time-points for
the transition of chondrocytes towards late differentiation events.
Conclusions: Our data indicate that long periods of continuous hydrostatic
pressure stimulate chondrocyte differentiation through a series of molecular
events involving the induction of SOX9 and RUNX2 and provide a theoretical
background for oro-facial functional orthopaedics.
Keywords:
Chondrocytes, Differentiation, Hydrostatic pressure, Sox9
