Unit:
Κατεύθυνση Ενδοδοντία (Κλινικές Ειδικεύσεις)Βιβλιοθήκη Οδοντιατρικής
Author:
Νικολουδάκη Γεωργία
Supervisors info:
Κοζυράκης Κωνσταντίνος, Χαμπάζ Μαρουάν, Τόσιος Κωνσταντίνος
Original Title:
Immunohistochemical study on the expression of Notch-1, Notch-2 receptors, Jagged-1 ligand and HERP-1 transcription factor on human periapical cysts.
Translated title:
Ανοσοϊστοχημική μελέτη της έκφρασης των υποδοχέων Notch-1, Notch-2, του συνδέτη Jagged-1 και του μεταγραφικού παράγοντα HERP-1 σε περιακρορριζικές κύστεις των γνάθων.
Summary:
Aim: Τhe immunohistochemical expression of Notch-1 and Notch-2 receptors,
Jagged-1 ligand and HERP1 transcription factor in periapical cysts was
evaluated and correlated with the presence of inflammation.
Materials and Methods: 30 formalin-fixed, paraffin-embedded human periapical
cysts were obtained and selected from archival biopsies submitted to the
Department of Oral Pathology and Medicine, Dental School, University of Athens.
The obtained sample was grouped according to the presence of inflammatory cell
in the underlying connective tissue infiltration were classified as
non-inflamed periapical cysts) (Orstavic & Mjor 1988) or inflamed periapical
cysts (>50% inflammatory infiltration). Immunohistochemistry was performed with
4 different antibodies against the above mentioned molecules using a fully
automated detection system. Statistical analysis was performed regarding the
presence of immunohistochemical staining in correlation with the degree of
inflammation in periapical cysts using Chi-square Test, Fisher Exact Test with
the Freeman-Halton extension; P < 0.05 was considered significant.
Results: Notch-1 was detected in the cytoplasm of the basal and suprabasal
epithelial cells of all periapical cysts (100%) and in some subepithelial
inflammatory cells. Presence of inflammation does not affect the expression of
Notch-1 in a statistically significant manner (Fishers Exact Test =1).
Expression of Notch-2 was detected in the cytoplasm of the basal and suprabasal
epithelial cells of nineteen periapical cysts (79.1%). Presence of
inflammation does not affect the expression of Notch-2 in a statistically
significant manner (x2=0.086, p=0.768) (Fisher exact test=1). Positive
immunostaining for Jagged-1 was detected in the basal and suprabasal epithelial
cells of 27 periapical cysts (90%). No apparent correlation was recognized
between Jagged-1 expression and the inflammatory cell infiltration of lining
epithelium in periapical cysts (x2=3.33, p=0.067, Fisher Exact test= 0.22). No
expression of HERP antibody could be detected in the lining epithelium of
periapical cysts, with the exception of its expression in some subepithelial
inflammatory cells
Conclusions: Notch pathway is an evolutionary conserved signaling mechanism
which regulated cell fates decisions during development and postnatal life. The
present observations further support the potential role of Notch signaling
pathway in the expansion and maintenance of the lining epithelium of periapical
cysts.
Keywords:
Periapical cysts, Periapical lesions, Notch, Jagged, Epithelium
Number of references:
374
