Κατεύθυνση Κλινική Χημεία
Library of the School of Science

2011-12-05

2011

Ρίζου Μυρτώ

Δημήτριος Παρασκευής Λέκτορας Ιατρική Σχολή ΕΚΠΑ, Ευρύκλεια Λιανίδου Καθηγήτρια Τμ. Χημείας ΕΚΠΑ, Χρήστος Κρούπης Επικ.Καθηγητής Ιατρική Σχολή ΕΚΠΑ

Ανάλυση της γενετικής ετερογένειας στελεχών του HIV-1: Μελέτη ανασυνδυασμού

Greek

HIV-1 originated in Africa via cross-species transmission from chimpanzees and
gorillas infected with simian immunodeficiency virus. In Europe HIV-1 infection
is dominated by subtype B transmission, however, non-B subtypes have been
increasingly reported. The characteristics of the HIV-1 epidemic in Greece are
somewhat unusual in comparison to those in most European countries since
subtype A was found to increase significantly in the last few years bearing the
consequent decrease of subtype B.
The present thesis studied the HIV-1 epidemic of subtype A1 in Greece as to the
possible existence of recombination in the gag gene of the virus.
We studied 25 subtype A samples from different risk groups and with different
dates of diagnosis of the disease. The first step was the isolation of the
virus RNA from the samples and the synthesis of the cDNA which was followed by
the amplification of the gag gene with the polymerase chain reaction using with
three different primer sets. Then DNA sequencing and subtype analysis of the
samples was performed in order to determine the existence of the recombination.
The results of the study showed that there is no recombination of the gag gene
in the studied samples.
Based on these results, the study and analysis of a larger number of samples is
suggested in order to confirm the finding of this thesis.

HIV, Recombination, Gag gene, DNA sequencing, Subtyping

Yes

15-21

Yes

70

124

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https://pergamos.lib.uoa.gr/uoa/dl/object/1315786