Supervisors info:
Μ. Κουππάρης Καθηγητής ΕΚΠΑ (Επιβλέπων), Κων. Λιάπης Ερευνητής Α’, Μ. Φ. Ι., Αν. Οικονόμου Αναπληρωτής Καθηγητής ΕΚΠΑ
Original Title:
Ανάπτυξη και επικύρωση μεθόδου για τον ταυτόχρονο προσδιορισμό φυτοπροστατευτικών προϊόντων, αντιβιοτικών και μυκοτοξινών με τη χρήση υγροχρωματογραφίας υδρόφιλων αλληλεπιδράσεων (hilic) συζευγμένης με φασματομετρία μαζών σε τρόφιμα φυτικής και ζωικής προέλευσης.
Translated title:
Μethod development and validation for the simultaneous determination of plant protection products, antibiotics and mycotoxins using hydrophilic interaction liquid chromatography (hilic) tandem mass spectrometry in foods of plant and animal origin.
Summary:
One of the recent trends in Analytical Chemistry is the development of
economic, quick and easy methods used in a field that includes analytes of
different classes and different physicochemical properties.
In this project a multi-residue method was developed for the simultaneous
determination of 26 xenobiotics (polar and hydrophilic) with hydrophilic
interaction liquid chromatography technique (Hydrophilic Interaction Liquid
Chromatography, HILIC) coupled with mass spectrometry technology, triple
quadrupole (LC-MS/MS) with ion source ESI. These analytes were the plant growth
regulators: chlormequat, daminozide, diquat, maleic hydrazide, mepiquat, the
insecticide: cyromazine, the ptu (metabolite of the fungicide propineb), the
antibiotics: chlorotetracycline, doxycycline, oxytetracycline (tetracyclines),
sulfamerazine, sulfadimidine, sulfamethoxypyridazine , sulfachloropyridazine,
sulfadimethoxine (sulfonamides), ciprofloxacin, danofloxacin, enrofloxacin,
flumequine, marbofloxacin, oxolinic acid (quinolones) and the mycotoxins:
aflatoxin B1, B2, fumonisin B1 and ochratoxin A. Additionally, a specific
method for the individual determination of the fungicide fosetyl-aluminium was
developed with ESI-.
The isolation of the analytes from the matrix is achieved with a fast and
effective technique. The validation of the multi-residue method was performed
at the levels: 10 μg/kg and 100 μg/kg in the following substrates:
fruits-vegetables (apples, apricots, lettuce and onions), cereals and pulses
(flour and chickpeas), animal products (milk and meat) and baby food. The
methods were validated according to the document SANCO/12495/2011 and show
satisfactory accuracy and precision. The linearity, accuracy, precision, along
with the limits of detection and the matrix factor were estimated. Finally,
there was an estimation for the methods’combined and expanded uncertainty for
each substance per substrate.
Keywords:
Hydrophilic Interaction Liquid Chromatography, HILIC, Mass Spectrometry, LC-MS/MS, Plant protection products: plant growth regulators, insecticides, fungicides, herbicides, Antibiotics, Mycotoxins
