Biocatalyst immobilized in a continuous flow system for the synthsesis of high added value products

Postgraduate Thesis uoadl:1316548 551 Read counter

Unit:
Τομέας Βοτανικής
Library of the School of Science
Deposit date:
2014-07-31
Year:
2014
Author:
Φιλίππου Κωνσταντίνα
Supervisors info:
Δημήτριος Χατζηνικολάου Επίκουρος Καθηγητής ΕΚΠΑ (Επιβλέπων), Κων/νος Βοργιάς Καθηγητής ΕΚΠΑ, Ανδρέας Ρούσσης Επίκουρος Καθηγητής ΕΚΠΑ
Original Title:
Βιοκαταλύτης ακινητοποιημένος σε σύστημα συνεχούς ροής για τη σύνθεση προϊόντων υψηλής προστιθέμενης αξίας
Languages:
Greek
Translated title:
Biocatalyst immobilized in a continuous flow system for the synthsesis of high added value products
Summary:
A continuous flow system is developed and proposed for the continuous synthesis
of various high value added products that interest food, cosmetics and
pharmaceutical industry. Such compounds can be esters of fatty acids or natural
phenolic acids or terpene alcohol esters. Four different continuous flow
systems were studied. The system that seemed to be more efficient was the one
that consisted of two columns filled with the enzyme-containing organogel and a
pump.
Further studies were carried out in order to optimize the reaction conditions.
A model reaction was used in order to optimize the system - the esterification
of lauric acid with 1-propanol. The reusability and storage (non-operational)
stability of the immobilized lipase were also studied. An alternative method is
proposed in order to minimize the reduction of enzymatic activity after three
uses of the same continuous flow system.
After system optimization three more reactions were studied. The esterification
reaction of p-hydroxy phenyl acetic acid with 1-octanol, the esterification
reaction of lauric acid with nerol and the esterification reaction of lauric
acid with geraniol. Finally, the products of the reactions mentioned above were
recovered from the reaction medium and propyl laurate has been identified by
NMR spectroscopy.
Keywords:
Biotransformations, Lipases, Esterification, Reactor, Organogel
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
185
Number of pages:
142
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