Unit:
ΠΜΣ Μικροβιακή ΒιοτεχνολογίαLibrary of the School of Science
Author:
Καρυόλαιμος Αλέξανδρος
Supervisors info:
Δημήτρης.Γ.Χατζηνικολάου Επίκ. Καθηγητής (Επιβλέπων),Γεώργιος Διαλλινάς Καθηγητής, Κωνσταντίνος Ε. Βοργιάς Καθηγητής
Original Title:
Βιοσύνθεση και απομόνωση επιλεγμένων κυκλικών πεπτιδίων σε καλλιέργειες βακτηριακών κυττάρων
Translated title:
Biosynthesis and isolation of cyclic peptides from bacterial cell cultures
Summary:
The Medical use of cyclic oligopeptides is of great significance, as it has
been consider that due to their structure degradation from proteases will not
occur. For example there have been many studies on the inhibitory activity of
cyclic peptides towards the aggregation of Aβ peptides in the Alzheimer
disease. Furthermore the manufacturing of small peptides and especially cyclic
oligopeptides, for many years has been a matter of Organic Synthesis making the
production costs high. In this project we propose the production of a variety
of cyclic oligopeptides from microorganisms, more specific from the model
system E.coli, a cost-effective and easy downstream process. The production was
carried out through a new technology of in vivo and in vitro cyclization of
proteins and peptides, called SICLOPPS (split intein circular ligation of
peptides and proteins). This process uses specially redesigned inteins
(inteins, intervening protein sequences), which have the capacity to function
as protein ligases and connecting the N-and C-termini of a protein or peptide
(extein, external protein sequence) with a stable peptide bond through an
autocatalytic reaction called protein splicing. The SICLOPPS system can produce
cyclic products whose size varies from large proteins to small oligopeptides.
Keywords:
Inteins, Cyclic peptides, Protein splicing, SICLOPPS, Extein
Number of references:
168
