Κατεύθυνση Κλινική Χημεία
Library of the School of Science

2013-07-10

2013

Ανδρέου Ειρήνη

Λεόντιος Κωστρίκης Καθηγητής Πανεπιστήμιο Κύπρου, Ευρύκλεια Λιανίδου Καθηγήτρια Τμήμα Χημείας ΕΚΠΑ , Δ. Παρασκευής Λέκτορας Ιατρική Σχολή ΕΚΠΑ

Γενετική ποικιλομορφία στελεχών της Ηπατίτιδας Γ που απομονώθηκαν απο μολυσμένους χρήστες ενδοφλέβιων ναρκωτικών στην Κύπρο

Greek

Genetic Variation of  HCV strains isolated from infected intravenous drug users in Cyprus

Hepatitis C (HCV) is a small, RNA enveloped virus belonging to the family
Flaviviridae, genus Hepacivirus, causing acute and chronic liver disease in
humans, including chronic hepatitis, cirrhosis and hepatocellular carcinoma.
In the present study examined the genetic diversity of patients with hepatitis
C virus (HCV) the period from August 2011 to October 2012. In this study 67
participants took part Injecting drug users who seek help detox the various
rehab centers on the island and which informed throughout the process that
would follow from the Assistant Director for Hepatitis. For the sampling each
potential service or guardian gave written consent to specific consent form to
participate in research. This form refers to the overall research program
called Genetic Studies in HCV strains from injecting drug users that has been
approved by the National Bioethics and which includes the research presented
here. Of the 67 samples, two samples were not sent to the lab. Then taken after
the plasma samples of 65 patients where sent sample and the isolated RNA
followed by amplification of the two regions CORE-E1 and NS5B by the method of
reverse transcription polymerase chain reaction (Nested RT-PCR), followed by
sequencing of the regions CORE-E1 and NS5B and finally held phylogenetic
analysis all seropositive samples (21 samples for each region) in regions
CORE-E1 and NS5B in HCV gene for determining the subtype. The phylogenetic
analysis of the sequences shown as the predominant subtype 3a with 16
sequences, 3 sequences of subtype 1b, one sequence of subtype 4d and one
sequence by recombination 2k/1b. By identifying the subtypes observed that
among HCV-infected injecting substances in Cyprus prevalent subtype 3a. Whith
Phylogenetic analysis of the group of patients who exercise intravenous drug
use, clustering observed between two patients suggesting a close relationship
epidemiological in these patients.
Finally we conclude that there is genetic heterogeneity of HCV virus but more
studies are needed to understand the evolutionary diversity of the epidemic and
the discovery of suitable vaccine and drug development to optimize treatment of
the virus.

HCV, DNA sequencing, Genetic diversity, Gene NS5B, Gene CORE-E1

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https://pergamos.lib.uoa.gr/uoa/dl/object/1316609