Genetic modification of fungus Fusarium oxysporum as to the phosphoketolase gene in order to create strains with increased ethanol production

Postgraduate Thesis uoadl:1316611 156 Read counter

Unit:
ΠΜΣ Μικροβιακή Βιοτεχνολογία
Library of the School of Science
Deposit date:
2015-11-11
Year:
2015
Author:
Ζήνωνος Ειρήνη
Supervisors info:
Μιλτιάδης Τύπας Καθηγητής
Original Title:
Γενετική τροποποίηση του μύκητα Fusarium oxysporum ως προς το γονίδιο της φωσφοκερολάσης με στόχο την δημιουργία στελεχών με αυξημένη παραγωγικότητα αιθανόλης
Languages:
Greek
Translated title:
Genetic modification of fungus Fusarium oxysporum as to the phosphoketolase gene in order to create strains with increased ethanol production
Summary:
This thesis is related to genetic modification of hyphomycetes Fusarium sp. to
increase the production of ethanol from lignocellulosic sources, as part of the
development effort consolidated bioprocessing systems. In particular, an
attempt was made to continuously expression of the endogenous phospoketolase
gene, under the regulation of the strong constitutive expression promoter gpdA,
at wild-type strain of F. oxysporum F3 and at the modified strain of F.
oxysporum FF11. At first, the plasmid vector pBsgentPK1 (8621 bp) was
constructed. The transformation of the wild type strain and the modified strain
FF11, with pBsgentPK1 vector, was attempted for introducing the
gpdA-PK-trpC-gnt section in the genomic DNA of the fungus. The colonies was
came up, were checked for their viability and two of them, F3.3 and FF11.56,
were studied further.These two strains, compared to F3 and FF11, were studied
for their growth on solid and liquid culture substrates of glucose, xylose and
arabinose, in anaerobic or microaerobic conditions. The result of the effort
for continuously expression of phosphoketolase gene, had a negative impact on
the sporiogenesis of modified strains, without even increasing the ethanol
production. Finally, despite the increase in biomass of the modified strains,
compared to F3 and FF11, in cultures with glucose substrate, ethanol production
was maintained at the same level, and there was an increase in the production
of acetic acid on the anaerobic growth phase, presence of arabinose as a
substrate.
Keywords:
F. oxysporum, Ethanol , Lignocellulosic , Phospoketolase
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
82
Number of pages:
100

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