Bacteria from tha area of Aspropirgos which can biodegrade crude oil

Postgraduate Thesis uoadl:1317219 212 Read counter

ΠΜΣ Μικροβιακή Βιοτεχνολογία
Library of the School of Science
Deposit date:
Κουταλιανού Μαρία
Supervisors info:
Καραγκούνη Αμαλία Καθηγήτρια (Επιβλέπουσα), Χατζηνικολάου Δημήτριος Επίκ. Καθηγητής, Διαλλυνάς Γεώργιος Επίκ. Καθηγητής
Original Title:
Ενδογενή βακτήρια της περιοχής του Ασπροπύργου με ικανότητα βιοεξυγίανσης
Translated title:
Bacteria from tha area of Aspropirgos which can biodegrade crude oil
Petroleum (L. petroleum, from Greek: πέτρα (rock) + Latin: oleum (oil)) is a
naturally occurring flammable liquid consisting of a complex mixture of
hydrocarbons of various molecular weights and other liquid organic compounds,
that are found in geologic formations beneath the Earth's surface. Petroleum is
used in manufacturing a wide variety of materials, and it is estimated that the
world consumes about 88 million barrels each day. The use of fossil fuels such
as petroleum can have a negative impact on Earth's biosphere, releasing
pollutants and greenhouse gases into the air and damaging ecosystems through
events such as oil spills.
A number of approaches and technologies have been developed for controlling oil
spills in marine shorelines and freshwater environments such as natural,
physical and chemical methods. Biodegradation of oil is one of the most
important processes involved in weathering and the eventual removal of
petroleum from the environment, particularly for the non volatile components of
petroleum. Numerous scientific review articles have covered various aspects of
this process and the environmental factors that influence the rate of
Taking into consideration the above mentioned it was studied the bioremediation
process at the area of Aspropirgos. One-hundred bacteria isolated from lake
Koumoundouro and the coastal area of Aspropirgos were screened for detection of
a n-alkane monoxygenase gene using degenerated primers developed to amplify the
alkB gene. The isolates carrying the alkB gene were further characterized by
16S rDNA gene sequencing. These isolates were able to grow in liquid cultures
with 5%crude oil as the sole carbon source. Finally, bacteria consortium were
made from these isolates and were studied for their ability to biodegrade oil
contaminated water.
Bioremediation, Biodegradation, Crude oil, Endogenous bacteria, Aspropirgos
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