Unit:
Κατεύθυνση Κλινική ΧημείαLibrary of the School of Science
Supervisors info:
Ευανθία Κασσή Καθηγήτρια
Original Title:
Επίδραση της 1,25 (OH)2 βιταμίνης D3 στην έκφραση παραγόντων που εμπλέκονται στην αποσταθεροποίηση της αθηρωματικής πλάκας: in vitro μελέτη σε ενδοθηλιακά κύτταρα
Translated title:
Effect of 1,25 (OH) 2 vitamin D3 on the expression of factors involved in the destabilization of atherosclerotic plaque: in vitro study in endothelial cells
Summary:
In the present study, we aimed to investigate the effect of vitamin D on the
expression of MMP-2 and MMP-9 and its inhibitors TIMP-1 and TIMP-2 as well as
the regulation of OPG expression in endothelial cells. Human aortic endothelial
cells (HAEC) were maintained in T-75 culture flasks at 370 C in a humidified
95% air-5% CO2 atmosphere. HAEC were grown in endothelial cell growth medium
M200 supplemented with 2% LSGS (low serum growth supplement. HAECs were
incubated with 1,25(OH)2D3 for 24, 48, 72 hours at a concentration range 10-8
– 10-11 in the in the absence of TNF-α or for 24 hours in the presence of
TNF-α at concentration 10ng/ml for the last 6 hours.
Although plaque rupture remains a major cause of plaque complications, other
mechanisms have emerged—that is, plaque erosion, and calcified nodules
protruding into the artery lumen
The present study revealed that 1,25(OH)2D3 at physiologically achievable
concentrations reduces the expression of MMP-9 and induces the expression of
TIMP-2 at mRNA level, in human aortic endothelial cells, implying a possible
protective role in the later stages of atheromatosis such as plaque
vulnerability.
Keywords:
Atherosclerosis, Vitamin D3, Human aortic endothelial cells, Plaque destabilization
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