Supervisors info:
Αναπληρώτρια Καθηγήτρια Α.Π.Θ. κα Σ. Γηρούση Α.Π.Θ. , Καθηγητής Α.Π.Θ. κ.Ι. Παπαδογιάννης, Καθηγήτρια Α.Π.Θ. κα Μ. Τσιμίδου
Summary:
The last decade, the scientific community has been trying intensively to
understand and explain the connection between ROS, DNA oxidative damage,
diseases and antioxidants. Electrochemical DNA biosensors are promising tools
for fast, inexpensive and simple in vitro analysis of free radicals and
antioxidants. Hydroxyl radicals (•OH) are known to damage DNA by oxidation of
the bases. These lesions are related to the aging process, cancer and
atherosclerosis. Fenton’s reaction is an important mediator of oxidative
damages in vivo. Antioxidants such as phenolic acids and plant extracts act as
free radical terminators and reduce the effect of the oxidative damage on dsDNA.
In the present work, we studied the electrochemical behavior of three
representative phenolic acids, caffeic acid, gallic acid and trolox using
cyclic voltammetry. We investigated the optimized conditions for their
determination (scan rate, deposition potential and time) using differential
pulse voltammetry. Moreover, we performed in vitro studies upon their effect on
the oxidative damage on dsDNA. We used a dsDNA modified carbon paste electrode,
a Pt wire counter electrode, a Ag/AgCl reference electrode and Fenton’s
mixture, as oxidative system. This biosensor has been applied as a screening
test to estimate the antioxidant capacity of aqueous herb extracts.
Keywords:
Phenolic compounds, Voltammetry, dsDNA electrochemical biosensor, Screening antioxidant test, Herbs
