Methodological study of the Limulus Amebocyte Lysate cascade (LAL test) on contact with flagellous protozoic parasites of genus Leishmania from liquid cell culture

Postgraduate Thesis uoadl:1318266 421 Read counter

Unit:
ΠΜΣ Μικροβιακή Βιοτεχνολογία
Library of the School of Science
Deposit date:
2016-07-22
Year:
2016
Author:
Παπαναστασίου Γεώργιος
Supervisors info:
Δημήτριος Χατζηνικολάου Επίκ. Καθηγητής
Original Title:
Μεθοδική μελέτη της αντίδρασης του Limulus Amebocyte Lysate cascade (LAL test) σε επαφή με μαστιγοφόρα πρωτοζωικά παράσιτα του γένους Leishmania από υγρή κυτταρο-καλλιέργεια
Languages:
Greek
Translated title:
Methodological study of the Limulus Amebocyte Lysate cascade (LAL test) on contact with flagellous protozoic parasites of genus Leishmania from liquid cell culture
Summary:
This research project is about a methodological study of the LAL mechanism
activation
on five flagellous protozoic parasites of genus Leishmania derived from Athens
Pasteur
institute. The experimental specimens (L. major, L. infantum, L. donovani, L.
tarantolae and L. donovani lacking LPG) were retrieved from plateau growing
phase
supernatant and both plateau and logarithmic growing phases pellet
reconstituted in
pyrogen free sterile water. Emphasis was given on the plateau growing phase
supernatant.
The LAL cascade activation leads to the activation of clotting enzyme which
transforms
the insoluble coagulogen to the soluble coagulin (Gel Clot) through two separate
activation paths:
1. C-B Factors: Bacterial gram-negative (LPS) activates LAL cascade.
2. G Factor: Fungal (1>3)-β-D-glucan, which acts as a structural component for
the majority of fungi, activates LAL cascade.
Plateau phase supernatant is of a great importance because the reaction was
stronger in
the supernatant and also because plateau phase is the pathogenic state of the
protozoic
parasites of this genus. The LAL mechanism was not activated through Factor-G
pathway. To the contrary, the LAL mechanism was activated through Factor-C
pathway
in all specimens. The reaction depended on the strain and the cell number.
Keywords:
Leishmaniasis, LAL-test, Reaction, Parasites, Diagnosis
Index:
Yes
Number of index pages:
7-8
Contains images:
Yes
Number of references:
34
Number of pages:
84
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