ΠΜΣ Μικροβιακή ΒιοτεχνολογίαLibrary of the School of Science
Δημήτριος Χατζηνικολάου Επίκ. Καθηγητής
Μεθοδική μελέτη της αντίδρασης του Limulus Amebocyte Lysate cascade (LAL test) σε επαφή με μαστιγοφόρα πρωτοζωικά παράσιτα του γένους Leishmania από υγρή κυτταρο-καλλιέργεια
Methodological study of the Limulus Amebocyte Lysate cascade (LAL test) on contact with flagellous protozoic parasites of genus Leishmania from liquid cell culture
This research project is about a methodological study of the LAL mechanism
on five flagellous protozoic parasites of genus Leishmania derived from Athens
institute. The experimental specimens (L. major, L. infantum, L. donovani, L.
tarantolae and L. donovani lacking LPG) were retrieved from plateau growing
supernatant and both plateau and logarithmic growing phases pellet
pyrogen free sterile water. Emphasis was given on the plateau growing phase
The LAL cascade activation leads to the activation of clotting enzyme which
the insoluble coagulogen to the soluble coagulin (Gel Clot) through two separate
1. C-B Factors: Bacterial gram-negative (LPS) activates LAL cascade.
2. G Factor: Fungal (1>3)-β-D-glucan, which acts as a structural component for
the majority of fungi, activates LAL cascade.
Plateau phase supernatant is of a great importance because the reaction was
the supernatant and also because plateau phase is the pathogenic state of the
parasites of this genus. The LAL mechanism was not activated through Factor-G
pathway. To the contrary, the LAL mechanism was activated through Factor-C
in all specimens. The reaction depended on the strain and the cell number.
Leishmaniasis, LAL-test, Reaction, Parasites, Diagnosis
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