Study of the activity of a thermophilic lipase in non-conventional systems

Postgraduate Thesis uoadl:1318586 404 Read counter

Unit:
ΠΜΣ Μικροβιακή Βιοτεχνολογία
Library of the School of Science
Deposit date:
2014-05-29
Year:
2014
Author:
Δελέντα Σταυρούλα
Supervisors info:
Δημήτριος Χατζηνικολάου Επίκ. Καθηγητής ΕΚΠΑ (Επιβλέπων), Κωνσταντίνος Βοργιάς Καθηγητής ΕΚΠΑ, Κοσμάς Χαραλαμπίδης Επίκ. Καθηγητής
Original Title:
Μελέτη της δραστικότητας μιας θερμόφιλης λιπάσης σε μη συμβατικά συστήματα
Languages:
Greek
Translated title:
Study of the activity of a thermophilic lipase in non-conventional systems
Summary:
The present study evaluated the action of the thermophilic lipase of a
Geobacillus strain in non-conventional systems. The term “non-conventional
systems” refers to: 1) the adjustment of High Hydrostatic Pressure HHP
(1,000-10,000 atm), 2) the use of Organic Solvents, in order to stabilize or
even increase the enzyme activity. Primarily, the enzyme purification required
the use of chromatography techniques (the enzyme had been already overexpressed
by the baculovirus flashBACTM system). The enzyme purity was then checked with
SDS-PAGE. The hydrophobic nature of lipase led to the enzyme immobilization and
to its further exploitation in this form. Esterification and
trans-esterification reactions took place in order to evaluate the enzyme in
applications of biotechnological interest. The results proved that lipase
activity increased with HHP application at 3,000 atm as also with enzyme
stabilization in organic solvents.
Keywords:
Lipase, Esterification, High hydrostatic pressure, Organic solvent, Enzyme purification
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
232
Number of pages:
[5], 114

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