Unit:
Κατεύθυνση Κλινική ΦαρμακευτικήLibrary of the School of Science
Author:
Καραγεωργιάδη Δήμητρα
Supervisors info:
Ανδρεάδου Ιωάννα Επίκ. Καθηγ.(Επιβλέπουσα), Μαχαίρας Παν. Καθηγ., Κυρούδη Σοφία Αναπλ. Καθηγ.
Original Title:
Μελέτη του μηχανισμού δράσης στατινών στη σταθεροποίηση των αθηρωματικών πλακών σε ασθενείς μετά από καρωτιδική ενδαρτηρεκτομή
Translated title:
Investigation of the mechanism of action of statins in the stabilization of atheromateous plaques
Summary:
The aim of our study is to investigate the mechanism of action of statins in
the stabilization of atheromateous plaques. We studied 29 random samples of
atherosclerotic plaques from patients who had undergone carotid endarterectomy.
One portion of the samples was prepared for immunohistochemistry for eNOS
expression and the rest was homogenized for further analysis of peNOS, pAkt,
their total forms and MDA. From the Western Blot analysis we observed greater
activation of eNOS and Akt in patients undergoing statin therapy, as well as an
increased activation of Akt in patients who had stable atherosclerotic plaques.
Immunohistochemistry showed weak expression of eNOS in unstable plaques. There
was no statistical importance in the MDA measurements. Concluding, Akt seems to
be more involved in the stabilization of atherosclerotic plaques compared to
eNOS. Plaque stabilization may not be the result of eNOS phosphorylation and NO
production, but the effect of Akt through another pathway. Furthermore, MDA
levels may contribute mainly at early stages of atherosclerosis and are not
associated with latter stages related to plaque vulnerability and disruption.
Keywords:
Statins, Atheromateous plaque, Oxidative stress, eNOS, Akt
Number of references:
103
