Supervisors info:
Μιλτιάδης Τύπας Καθηγητής (Επιβλέπων), Κοσμάς Χαραλαμπίδης Επικ. Καθηγητής, Δημήτρης Χατζηνικολάου Επικ. Καθηγητής
Summary:
This research was concerned with the study of the properties of ligninolytic
basidiomycetes, due to a variety of enzyme activities, resulting in degradation
tough polymers, such as lignin, which is the second most abundant natural
polymer, particularly important in the carbon cycle. Specifically in this paper
used the white rot fungus Pleurotus ostreatus and focused on the study of some
of the enzymes that confer the ability of lignin degradation, which included
the family of laccases. The laccases catalyze the oxidation in a range of
aromatic substrates with concomitant reduction of molecular oxygen to water. No
particular exhibit specificity to the substrate, thus having the ability to
degrade many natural and complex aromatic compounds, usually as waste
processing. With these properties laccases can be used in various fields of
industry, nutrition and bioremediation.
In this research, it was attempted to study of this family in different levels.
• Bioinformatics analysis of gene family.
• Attempt targeted gene inactivation laccases of P. ostreatus,
• Attempt heterologous expression of eukaryotic laccases of P. ostreatus, in
the white rot fungus, Phanerochaete chrysosporium.
• Attempt prokaryotic heterologous expression of laccase of P. ostreatus in
Escherichia coli.
Keywords:
Laccases, Lignin, Basidiomycetes, Pleurotus ostreatus, Phanerochaete chrysosporium
