Summary:
The purpose of this study consists of two parts: The first one is the isolation
of total lipids of farmed Sea Bream (Sparus aurata) by the appropriate
extraction methods and their separation to total polar and total neutral lipid
fractions. Subsequently, the total polar lipid fractions were further separated
by normal-phase High-performance Liquid Chromatography (HPLC) and then were
tested in vitro for their ability to either induce platelet aggregation or
inhibit the PAF induced washed rabbit platelet aggregation, who is considered
to be the main cause of atherogenesis, in order to specify compounds with
interesting biological activity. The fractions which showed the strongest
aggregatoty activity were further separated by reverse-phase HPLC, so that be
obtained potentially more highly purified polar lipid fractions. The biological
property of these polar fractions was exanimated as above. The polar lipid
fractions which showed the most remarkable aggragatory activity are able to be
tested by chemical specifications and the application of Mass Spectrometry (MS)
in order to determine their conceivable chemical structure.
The second part of this study comprises the effort of enrichment of fish feed
which is used at aquacultures, by replace the fish oil with plant oil and
especially with biological active polar lipids of olive pomace oil. The aim is
to produce fish feed and consequently fishes with improved cardio-protective
properties. For this reason, total polar lipids of both enriched and
conventional fish feed were extracted and isolated by the same methods, as
happened with the fish, and their biological activity to induce the PAF
platelet aggregation was tested and compared. In this study, the enrichment of
conventional aquaculture fish feed by replacing fish oils with OP was achieved
and evaluates the notable improved bioactive properties of polar lipid
fractions of enriched fish feed to induce the platelet aggregation which is
caused by PAF-agonists, which indicates the successful transport of biological
components of pomace olive oil to fish feed and consequently to fish flesh.
Keywords:
Fish oil, Fish feed, Olive pomace, Cardioprotective properties, Platelet activating factor
