Μετα-ανάλυση μελετών αξιολόγησης αυτοματοποιημένων μικροβιολογικών συστημάτων: σύγκριση της απόδοσης των οργάνων Phoenix (Becton Dickinson) και Vitek 2 (bioMerieux) ως προς την ορθή ταυτοποίηση των gram θετικών και gram αρνητικών βακτηρίων

Postgraduate Thesis uoadl:1318891 314 Read counter

Unit:
Τομέας Βιολογίας Κυττάρου Και Βιοφυσικής
Library of the School of Science
Deposit date:
2012-01-15
Year:
2012
Author:
Χατζηγεωργίου Καλλιόπη-Σταυρούλα
Supervisors info:
Kαθηγητής Σταύρος Χαμόδρακας, Eπίκουρος Kαθηγητής Παντελής Μπάγκος (Επιβλέπων), Λέκτορας Βασιλική Οικονομίδου
Original Title:
Μετα-ανάλυση μελετών αξιολόγησης αυτοματοποιημένων μικροβιολογικών συστημάτων: σύγκριση της απόδοσης των οργάνων Phoenix (Becton Dickinson) και Vitek 2 (bioMerieux) ως προς την ορθή ταυτοποίηση των gram θετικών και gram αρνητικών βακτηρίων
Languages:
Greek
Summary:
Phoenix 100 and Vitek 2 systems are commonly used in hospital laboratories for
rapid identification of microorganisms. The present meta-analysis aims to
evaluate and compare their performance on gram positive and gram negative
bacteria. MEDLINE database was searched up to October 2010 for the retrieval of
relevant articles. Pooled correct identification rates were derived from
random-effects models, using the arcsine transformation. Bivariate
random-effects meta-analysis was applied, where appropriate, to compare the
performance of the systems. Separate analyses were conducted at the genus and
species levels; subanalyses and meta-regression were undertaken to unveil
meaningful system- and study- related modifiers. A total of 29 (6,635 isolates)
and 46 (11,223 isolates) articles were eligible for Phoenix and Vitek 2,
respectively. Overall, Phoenix exhibited better performance than Vitek 2 at
both genus (97.70% versus 95.20%, p=0.092) and species (92.51% versus 82.90%,
p=0.002) levels, consistently confined to studies employing conventional
reference methods and Vitek 2 fluorescent cards. However, direct comparison of
the instruments, based on 11 reports, did not demonstrate any significant
difference (95.20% versus 92.58%, p=0.540 for genus and 87.54% versus 87.52%,
p=0.422 for species). Subanalyses and meta-regression suggested that the
comparator method, type of Vitek 2 card, and relative frequencies of pathogens
significantly affected the performance of the systems. In conclusion, the
accuracy of both Phoenix and Vitek 2 seems modified by underlying system-,
sample-, and comparator method-related parameters. Future simultaneous
assessment of the instruments against molecular comparator procedures may
facilitate interpretation of the current observations.
Keywords:
Meta-analysis, Identification, Bacteria, Phoenix, Vitek 2
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
111
Number of pages:
180
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