Unit:
Κατεύθυνση Ανόργανη Χημεία και ΤεχνολογίαLibrary of the School of Science
Supervisors info:
Χριστιάνα Μητσοπούλου Καθηγήτρια
Original Title:
Μεταλλικές και Υβριδικές Νανοδομές Χρυσού και Σιδήρου για την Διάγνωση και Θεραπεία του Καρκίνου: Σύνθεση, Χαρακτηρισμός και Βιολογική Αξιολόγηση
Translated title:
Metallic and Hybrid Nanostructures of Gold and Iron for the Diagnosis and Therapy of Cancer: Syntesis, Characterization and Biological Evaluation
Summary:
The purpose of this project is the synthesis, characterization and biological
evaluation of metallic and hybrid nanostructures, for bioapplications in
diagnosis and treatment of cancer.
The metallic nanostructures under investigation are gold nanoparticles and iron
oxide nanoflowers. The gold nanoparticles were synthesized according to the
classical Turkevich method by testing different reducing agents that could
simultaneously act as capping agents. Those agents were trisodium citrate and
L-amino acids; aspartic acid, lysine, serine, asparagine, valine and threonine.
Structural and toxicological evaluation was performed among the different
resulting gold nanostructures, while their potential as contrast agents was
examined upon MCF7 breast cancer cells. The iron oxide nanoflowers were
synthesized by polyol thermal decomposition and were further functionalized
with trisodium citrate in order to increase their biocompatibility. Their
magnetic and thermal response was also investigated.
The hybrid nanostructures under study include the hollow polymer spheres
embedded with gold nanoparticles and the thermosensitive liposomes with
encapsulated gold or iron oxide nanoparticles. These hybrid nanostructures can
be used as drug delivery systems for cancer therapy. For this purpose, their
loading capacity and/or encapsulation efficiency of two drugs, doxorubicin and
bleomycin, at the same time, was investigated. Furthermore, a viability study
was conducted upon MCF7 breast cancer cells, in order to determine their
behavior in the absence or presence of drugs. The hollow polymeric nanospheres
embedded with gold nanoparticles were also tested for their efficacy as
contrast agents with confocal microscopy on the same cell line.
Keywords:
Gold Nanoparticles, Iron Oxide Nanoflowers, Liposomes, Hollow Polymeric Nanospheres, Cancer
File:
File access is restricted.
document.pdf
5 MB
File access is restricted.
