Microbial and photochemical production of squalene peroxides and method development for their quantitative analysis, using 2D qNMR.

Postgraduate Thesis uoadl:1319045 300 Read counter

Unit:
ΠΜΣ με ειδίκευση ΑΠΟΜΟΝΩΣΗ, ΑΝΑΠΤΥΞΗ, ΠΑΡΑΓΩΓΗ ΚΑΙ ΕΛΕΓΧΟΣ ΒΙΟΔΡΑΣΤΙΚΩΝ ΦΥΣΙΚΩΝ ΠΡΟΪΟΝΤΩΝ
Library of the School of Science
Deposit date:
2015-11-26
Year:
2015
Author:
Παπαδήμου Χρυσάνθη
Supervisors info:
Μαγιάτης Προκόπιος Αναπλ.Καθηγητής.(Επιβλέπων), .Βελεγράκη Αριστέα Επίκ. Καθηγήτρια, Χήνου Ιωάννα Καθηγήτρια
Original Title:
Μικροβιακή και φωτοχημική παραγωγή υπεροξειδίων του σκουαλενίου και ανάπτυξη μεθόδου ποσοτικού προσδιορισμού τους με 2D qNMR.
Languages:
Greek
Translated title:
Microbial and photochemical production of squalene peroxides and method development for their quantitative analysis, using 2D qNMR.
Summary:
The aim of the present thesis was to study how Malassezia fungi participate in
squalene peroxides’ (SqmOOH) production and to examine differences in SqmOOH
production possibly occurring among species and species strains.
SqmOOH from Malassezia fungi cultures were extracted and quantified. SqmOOH
photochemical production was examined, followed by their isolation and
respective quantification. A method for squalene and SqmOOH quantification was
developed, using 2D qNMR, and was applied in complex mixtures.
The method developed is simple, short and minimally interfering with the
sample. The method was used for microbial and chemical samples giving the
following results: Different Malassezia strains affect SqmOOH production in a
quantitatively different and time-dependent manner. 6-FICZ – an indole
derivative, Malassezia metabolite and nanomolecular photosensitizer – under UVA
radiation acts as a catalyst for SqmOOH production.
Keywords:
Squalene peroxides, Photo-oxidation, Malassezia, 6-FICZ, 2D qNMR
Index:
Yes
Number of index pages:
118-121
Contains images:
Yes
Number of references:
144
Number of pages:
130
File:
File access is restricted.

document.pdf
6 MB
File access is restricted.