Molecular and functional characterization of At5g48310 gene during the growth and development in plants Arabidopsis thaliana.

Postgraduate Thesis uoadl:1319114 123 Read counter

Unit:
ΠΜΣ Μικροβιακή Βιοτεχνολογία
Library of the School of Science
Deposit date:
2013-08-01
Year:
2013
Author:
Μαρτζίκου Μαρία
Supervisors info:
Κοσμάς Χαραλαμπίδης Επίκ. Καθηγητής ΕΚΠΑ (επιβλέπων), Αμαλία Καραγκούνη Καθηγήτρια ΕΚΠΑ, Ανδρέας Ρούσσης Επίκ. Καθηγητής ΕΚΠΑ
Original Title:
Μοριακός και λειτουργικός χαρακτηρισμός του γονιδίου At5g48310, κατά τη διάρκεια ανάπτυξης φυτών Arabidopsis thaliana.
Languages:
Greek
Translated title:
Molecular and functional characterization of At5g48310 gene during the growth and development in plants Arabidopsis thaliana.
Summary:
The molecular basis and the preservation mechanisms of plant osmosensing
remains unknown.The gene At5g48310of the plant Arabidopsisthaliana was
molecularly and functionally studied. This is one of the genes for which there
is no reference in the bibliography. It is a gene of unknown function but here
is made an early attempt for its characterization. It has specific domains
which suggest that it is related with the sugar transfer. Its homolog, Ze173,
is a very good index during the growth of its supply system. The phylogenetic
analysis of the protein At5g48310 has shown its grouping to a common subgroup
with other uncharacterized proteins. Using computational programs we show that
it is probably localized in the plasmatic membrane. Analysis of transgenic
plants reveals that At5g48310is expressed during reproductive development,
predominantly in the inflorescence and also in whole vascular system of the
stem, while overexpression of At5g48310 does not lead to any phenotypic changes
during growth and development. It is also observed great expression of the gene
to the chemical isoxaben. Homozygous mutants are of total loss of function
(knock-out). As a result, a change in the topology of the protein at the
membrane blocks isoxaben’s binding. Since the protein At5g48310 is localized in
the membrane, it maybe changes the permeability and allows water loss. So we
experimentally shown that the homozygous plants are sensitive to drought and
resistant to isoxaben.
Keywords:
A.thaliana, At5g48310, Drought, Isoxaben, Sugars
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
103
Number of pages:
102
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