Supervisors info:
Βασίλειος Ρούσσης Καθηγητής, Όλγα Τζάκου Καθηγήτρια , Ευσταθία Ιωάννου Επίκ. Καθηγήτρια (Επιβλέπουσα)
Original Title:
Προϊόντα βιομετατροπής του 14-οξο-3,7,18-δολαμπελλατριενίου από ένα ενδοφυτικό στέλεχος Bacillus megaterium θαλάσσιας προέλευσης
Translated title:
Biotransformation of 14-oxo-3,7,18-dolabellatriene by a marine derived endophytic strain Bacillus megaterium.
Summary:
In the framework of the present thesis 11 endophytic bacterial strains were
isolated from samples of the brown alga Dilophus spiralis which was collected
in Elafonissos. In parallel, the major metabolite, (1R,3E,7E,11S,12S
)-14-oxo-3,7,18-dolabellatriene, was isolated in sufficient amounts from the
organic extract of the alga.
Subsequently, the 11 bacterial strains were evaluated for their ability to
biotransform (1R,3E,7E,11S,12S)-14-oxo-3,7,18-dolabellatriene. Strain Bacillus
megaterium BI0223, which was shown to successfully bioconvert the specific
metabolite, was cultivated in larger scale liquid cultures supplemented with
(1R,3E,7E,11S,12S)-14-oxo-3,7,18-dolabellatriene.
The organic residues of the bacterial cultures were subjected to a series of
chromatographic separations that led to the isolation and structure elucidation
of eight biotransformation products. The structures of the isolated natural
products were established by means of spectroscopic analyses, including 1D and
2D NMR experiments, as well as MS data. Among the isolated metabolites, seven
are new natural products.
Keywords:
Dilopus spirallis, Bacillus megaterium, Biotransformations, Dolabellanes
