Determination of fat-soluble vitamins in inafant formulae and baby foods by HPLC

Postgraduate Thesis uoadl:1319633 579 Read counter

Unit:
Κατεύθυνση Χημεία Τροφίμων
Library of the School of Science
Deposit date:
2016-10-17
Year:
2016
Author:
Κάτσα Μαρία
Supervisors info:
Προεστός Χαράλαμπος Επίκ. Καθηγητής
Original Title:
Προσδιορισμός των λιποδιαλυτών βιταμινών σε βρεφικές και παιδικές τροφές με Υγρή Χρωματογραφία Υψηλής Απόδοσης
Languages:
Greek
Translated title:
Determination of fat-soluble vitamins in inafant formulae and baby foods by HPLC
Summary:
This study aimed at developing a method for the determination of fat-soluble
vitamins to be used in routine analytical work. The purpose of the method was
to be accurate, fast and inexpensive. It was applied in a very specific matrix
and that was the infant formulas and baby foods which are very important for
sensitive categories of consumers such as infants and young children. The
samples selected were products sold commercially by the Yiotis company on the
Greek market. A comparison was conducted between the analytical results of this
method and those indicated on the labels of the products for vitamins A, D, E
and K. The qualitative and quantitative determination of vitamins was performed
by High Performance Liquid Chromatography (HPLC) and diode array detector
(DAD).More specifically, a development and optimization of the saponification
step, extraction step and the isolation of the vitamins from the samples were
realized and indicatively was studied the time saponification, the use of
antioxidants and the number of extractions combined with the extraction time.
The proposed method was validated as far as the detection and quantification of
vitamins A and E are concerned. It was proved reliable with results into the
permitted limits and close to the indications of sample’s labels. At the same
time is in this study a fast method for the qualitative determination of
vitamins is presented omitting the step of saponification (screening method).
In the future, these methods may be further researched towards the
determination of all the fat-soluble vitamins on other foods such as cereals,
cocoa, chocolate
Keywords:
Vitamin A, Vitamin E, Infant formulas, HPLC, Saponification
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
143
Number of pages:
155
document.pdf (2 MB) Open in new window