Supervisors info:
Προεστός Χαράλαμπος Επίκ. Καθηγητής (Επιβλέπων), Μαρκάκη Παναγιώτα Αναπλ. Καθηγήτρια , Ζουμπουλάκης Παναγιώτης Ερευνητής Γ Ινστιτούτου Βιολογίας, Φαρμακευτικής Χημείας και Βιοτεχνολογίας Εθνικό Ίδρυμα Ερευνών
Summary:
This is a comperative study and conducted in cooperation of the National
University of Athens (UOA), the National Hellenic Research Foundation and the
Food Technology Department of the Technoligocal Educational Institute of Athens
in order to put under the scope infusions and decoctions of 10 Greek herbal
species, namely Matricaria chamomilla, Echinacea purpurea, Lippia citriodora,
Menta pulegium, Mentha spicata, Origanum majorana, Origanum vulgare, Thymus
capitatus, Hypericum perforatum and Hippophae rhamnoides. This study implements
NMR metabolomics, HPLC/Ms analysis and spectrophotometric studies. The primary
objective was to determine whether the metabolic profile of each preparation is
capable to characterize it and what exactly indicate. Moreover, the antioxidant
and antiradical profiles of the preparations were assessed with the DPPH, FRAP
and the ABTS assays, while the Folin-Ciocalteu assay evaluated the total
phenolic content of the different preparations. PCA-class and OPLS-DA models
highlighted the differences among the samples and pinpointed specific classes
of compounds for each preparation. Both the phenolic profile, as expressed by
the three analytical methods and the antiradical capacity, evidenced by higher
prices in infusions compared to beverages.The time factor was examined for the
preparation of decoctions and the 15 min preparation was generally found rich
in phenols of higher antioxidant capacity which could be attributed to further
release of the aglycon structures due to extended heat treatment.
Keywords:
Herbs, Antioxidant, NMR metabolomics, HPLC-MS, Phenolic content
