Supervisors info:
Κωνσταντίνος Μεθενίτης Αναπλ.Καθηγητής (Επιβλέπων), Χριστίνα-Άννα Μητσοπούλου Καθηγήτρια, Μαρίνα Σαγνού Ερευνήτρια Γ' ΕΚΕΦΕ Δημόκριτος
Summary:
In the current thesis, the characterization of a new curcumin derivative (4,
4΄- ((1E, 1΄E)
-(1-(pyridin-2-yl)-1H-pyrazole-3,5-diyl)bis(ethene-2,1-diyl))bis(2-methoxyphenol
)) and the synthesis and the characterization of the corresponding copper (II)
complex were described. We also studied their DNA-binding properties with a
range of techniques, as well as the in vitro cytotoxicity studies against human
cancer cell lines ΜDA-MB 231 (human breast) and U251 (glioblastoma).
In this innovative derivative, the group of β-diketone of curcumin was replaced
by the moiety of the pyridine-substituted pyrazole, aiming both at the
improvement of the natural product’s properties, such as the low solubility and
stability in water at acidic and neutral pH, its low selectivity and
bioavailability, and the ability of the complexation with metals. This new
derivative of curcumin was synthesized successfully and it was used as a NN
ligand in the coordination sphere of the copper complex. Subsequently, their
interaction with CT-DNA. The results of this study indicate the ability of the
ligand and the complex to interact with DNA, with a stronger DNA interaction
with the complex than the ligand. In vitro cytotoxicity studies against ΜDA-MB
231 and U251 were determined using MTT assay.
Keywords:
Curcumin, Curcumin’s derivatives, Copper, DNA, Anticancer activity
