Κατεύθυνση Επιστήμη Πολυμερών και Εφαρμογές τηςLibrary of the School of Science
Χατζηχριστίδη Μαργαρίτα Επίκουρη Καθηγήτρια Ε.Κ.Π.Α.
Σχηματοποίηση βιομορίων με χρήση φθοριωμένων πολυμερών και υδροφθοροαιθέρων ως διαλύτες
Biomolecules patterns using a fluorinated photoresist and HFEs as polymer solvents
During the last years, there is an increasing interest in creating patterns of
functional biomolecules on surfaces for applications such as biosensors,
biological microelectromechanical systems (bio-MEMs), and microfluidic devices.
Fluorinated polymers and solvents are chemically orthogonal to organic
materials such as polymeric semiconductors, and provide the perfect material
system to develop new patterning approaches. Conventional photolithography,
although is a mature patterning technique that allows high-resolution, precise
alignment, and is a high-throughput process has the disadvantage of requiring
harsh developers and exposure conditions as well as elevated temperatures,
which often lead to denaturation of delicate biomolecules. Hydrofluoroethers
are nontoxic and environment friendly solvents, as being chemically benign to
nonfluorinated organic electronic materials. In addition, HFEs are orthogonal
solvents to many organic materials, that is, the organic materials are
insoluble and do no swell when immersed in HFEs.
In the present work we developed an approach to create biomolecules patterns
using a fluorinated photoresist and different HFEs as polymer solvents and
developers, respectively. At first, proteins were patterned by physical
adsorption in negative tone polymer. Then, proteins were patterned by chemical
bonding in negative tone polymer. Finally, the negative tone polymer change
into positive and then proteins were patterned by physical adsorption.
Photolithography, Microarrays, Fluorinated polymers, Hydrofluoroethers, Proteins
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