Surfacemodificationf or covalent attachment of biomolecules via photochemistry of azides or click chemistry

Postgraduate Thesis uoadl:1320492 274 Read counter

Unit:
Κατεύθυνση Επιστήμη Πολυμερών και Εφαρμογές της
Library of the School of Science
Deposit date:
2014-09-03
Year:
2014
Author:
Βρεττού Φωτεινή
Supervisors info:
Χατζηχρηστίδη Μαργαρίτα Λέκτορας ΕΚΠΑ (Επιβλέπουσα), Πιτσικάλης Μαρίνος Αναπλ. Καθηγητής ΕΚΠΑ, Πέτρου Παναγιώτα Α΄ΕΚΕΦΕ Δημόκριτος
Original Title:
Τροποποίηση επιφανειών με πολυμερή για την ομοιοπολική πρόσδεση βιομορίων με φωτοχημεία αζιδίων ή χημεία <<κλικ>>
Languages:
Greek
Translated title:
Surfacemodificationf or covalent attachment of biomolecules via photochemistry of azides or click chemistry
Summary:
This thesis purpose is the development of suitable processes for the
modification of silicon surfaces for covalently bonding of biomolecules via
azide photochemistry or “click” chemistry. Click chemistry is the selective
reaction between two small chemical units, the main advantage of which is that
the reaction takes place regardless of the molecules they are attached to. An
example is the click chemistry between azide and alkyne groups. In this study
the “click” groups used were azide and dibenzylcyclooctyne.
In the “click” chemistry study, the materials investigated for the surface
modification of silicon wafers were self-assembled monolayers (SAMs) terminated
with an epoxy ring or an amino group, and a lithographic epoxy resin.
Initially, the above materials were coated on the surface of the silicon
wafers, and the films were then covalently bonded to biotin-derivatives or
other molecules which bore a suitable group for subsequent "click" chemistry.
In each case the degree of immobilization of the molecules on the surface was
studied via "click" chemistry of the modified surface with a suitable
fluorescent substance modified with the complementary to the surface "click”
group.
The azide photochemistry approach was studied using silicon surfaces modified
by SAMs ending in double bonds. The photochemical binding of molecules bearing
azides was then attempted in those substrates.
Keywords:
Azide photochemistry, Click chemistry, Surface modification, Self- assembling monolayers, Epoxy-based resists
Index:
Yes
Number of index pages:
131,132,133,134
Contains images:
Yes
Number of references:
135
Number of pages:
153

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