Pharmacological investigation of growth and ageing mechanisms in S. cerevisiae

Postgraduate Thesis uoadl:1320628 238 Read counter

Unit:
Διατμηματικό ΠΜΣ Εφαρμογές της Βιολογίας στην Ιατρική
Library of the School of Science
Deposit date:
2015-01-09
Year:
2015
Author:
Κακολύρη Μαρία
Supervisors info:
Αικατερίνη Τυλιγάδα Αναπλ. Καθηγήτρια
Original Title:
Φαρμακολογική διερεύνηση των κυτταρικών μηχανισμών ανάπτυξης και γήρανσης στον Saccharomyces cerevisiae
Languages:
Greek
Translated title:
Pharmacological investigation of growth and ageing mechanisms in S. cerevisiae
Summary:
The yeast Saccharomyces cerevisiae is a eukaryotic unicellular organism which
is commonly used as an experimental model for the study of numerous biological
processes, including ageing. Its wide use is mainly due to the highly conserved
molecular pathways and the homology of genes between yeasts and mammals. The
aim of this study was to investigate a combination of parameters that
characterize eukaryotic growth and ageing, using S. cerevisiae as an
experimental organism, as well as the effects of dimethylsulfoxide (DMSO) and
histamine on these parameters. The experimental approach included long-term
incubation of asynchronous yeast cultures in the absence or presence of
pharmacologically active substances administered at various time points. The
determination of parameters characterizing growth and ageing, such as cell
proliferation, viability, division, colony forming potential and morphology was
performed at defined intervals during growth. The results showed that histamine
induces no detectable effects on these parameters. On the contrary, DMSO
induced dose-dependent and phase of growth-independent inhibition of cell
proliferation, acceleration of the onset of the aged phenotype and
epigenetic-like phenotype in yeast cultures. Histamine co-administration
partially reversed the inhibitory effect of DMSO. Overall, the findings support
the use of yeast as an experimental model to study ageing. In parallel, they
provide the lead for further investigating the putative effect of DMSO on
ageing and in the long-term for identifying new potential pharmacological
targets for ageing.
Keywords:
Ageing, Yeast, DMSO, Histamine, Pharmacology
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
137
Number of pages:
vi, 122
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