Unit:
Κατεύθυνση Χημική Ανάλυση-Έλεγχος ΠοιότηταςLibrary of the School of Science
Author:
Χατζηιωάννου Αναστασία - Χρυσοβαλάντου
Supervisors info:
Γεώργιος Θεοδωρίδης Αναπλ. Καθηγητής (Επιβλέπων), Ιωάννης Ν. Παπαδογιάννης Καθηγητής, Ελένη Γκίκα Λέκτορας
Original Title:
Φασματοσκοπία πυρηνικού μαγνητικού συντονισμού στην ανάλυση μεταβολικού προφίλ βιολογικών δειγμάτων αμνιακού υγρού και ορού αίματος
Translated title:
Nuclear magnetic resonance spectroscopy analysis of the metabolic profile of amniotic fluid and blood serum
Summary:
Recent metabonomics’ studies of maternal blood and fetal biological fluids have
expanded our knowledge concerning the interchange between the metabolism of
fetus and its mother. Nevertheless, hitherto no study has examined the human
amniotic fluid (HAF) composition in relation with the maternal habitual diet.
The present study aimed to identify correlations between maternal dietary
habits and metabolic profiles of HAF and blood serum derived from the NMR
analysis.
Samples were obtained from 29 women that underwent amniocentesis for prenatal
diagnosis (17th - 21th gestational week). Dietary assessment was carried out by
qualified registered dietician using a food frequency questionnaire and 24-h
recall. The holistic analysis of all samples was accomplished utilizing a 600
MHz Varian NMR spectrometer by applying CPMG pulse sequence and using sodium
maleate as internal standard. Sample pretreatment included freeze-drying to
increase signal intensities.
PLS models were created in order to detect correlations between the metabolic
profile of HAF and the gestational week of amniocentesis, the maternal
pre-pregnancy BMI, as well as the maternal dietary profile. Thereafter, PCA and
OPLS-DA models managed to discriminate samples and metabolites responsible for
the observed differentiations were identified.
Keywords:
Nuclear magnetic resonance spectroscopy, Amniotic Fluid, Blood serum, Metabolic profile, Metabonomics
Number of pages:
VIII, 98
