Molecular and phenotypic analysis of developmental genes of Arabidopsis thaliana

Postgraduate Thesis uoadl:1321272 251 Read counter

Unit:
ΠΜΣ Μικροβιακή Βιοτεχνολογία
Library of the School of Science
Deposit date:
2013-04-19
Year:
2013
Author:
Μπερή Δέσποινα
Supervisors info:
Κοσμάς Χαραλαμπίδης Επίκ. Καθηγητής
Original Title:
Molecular and phenotypic analysis of developmental genes of Arabidopsis thaliana
Languages:
English
Translated title:
Μοριακή και Φαινοτυπική ανάλυση αναπτυξιακών γονιδίων του Arabidopsis thaliana
Summary:
The WD40 protein superfamily is extremely widespread in eukaryotic organisms.
So far, more than thirty distinct functions have been identified to involve
WD40 proteins, from fungi to human and plants. Because of their ability to
fold into a β- propeller structure, WD40 proteins can act as scaffolds for
protein-protein and protein-DNA interactions. In plants such proteins have been
identified as putative subunits of E3 Cullin-RING Ubiquitin Ligases complexes
(CRLs). Through ubiquitynation, they can regulate processes that affect almost
every aspect of plants’ life including the cell cycle, hormonal signaling,
anthesis and cell patterning. In this study, we have focused on the molecular
and functional characterization of the AtULC1 gene from Arabidopsis thaliana,
encoding a putative E3 CRL subunit. In contrast to other characterized E3 CRL
subunits, which contain several functional domains, AtULC1 belong to a less
characterized sub-family of proteins comprising solely of WD40 domains. Several
lines of evidence, which are based on the phylogenetic analysis, the specific
expression pattern, the dual subcellular localization and the phenotypic
analysis of ulc1-i knock-down mutant lines, imply that AtULC1 may regulate,
through an auxin dependend ubiquitination pathway, crucial developmental
processes, such as proper development and function of the Shoot Apical Meristem
(SAM), apical dominance and fertilization.
Keywords:
WD40 proteins, Ubiquitin, Phyllotaxis, E3 ligases, Auxin
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
94
Number of pages:
105

document.pdf
18 MB
File access is restricted.