Application of cyclic and static tensile mechanical loading of human periodontal ligament fibroblasts

Postgraduate Thesis uoadl:1325927 356 Read counter

Unit:
Βιβλιοθήκη Οδοντιατρικής
Κατεύθυνση Ορθοδοντική (Κλινικές Ειδικεύσεις)
Deposit date:
2016-12-12
Year:
2016
Author:
Eliades Anna
Supervisors info:
Ιωσήφ Σηφακάκης, Επίκουρος Καθηγητής, Τμήμα Οδοντιατρικής, ΕΚΠΑ
Ηλίας Μπιτσάνης, Επίκουρος Καθηγητής, Τμήμα Οδοντιατρικής, ΕΚΠΑ
Απόστολος Τσολάκης, Επίκουρος Καθηγητής, Τμήμα Οδοντιατρικής, ΕΚΠΑ
Original Title:
Εφαρμογή στατικών και κυκλικά επαναλαμβανόμενων δυνάμεων τάσης σε καλλιέργειες ανθρωπίνων ινοβλαστών του περιρριζίου
Languages:
English
Translated title:
Application of cyclic and static tensile mechanical loading of human periodontal ligament fibroblasts
Summary:
Periodontal ligament (PDL) is continuously exposed to different types of mechanical forces, such as cyclic or static deformation, caused by occlusion and mastication as well as during orthodontic treatment. These stresses activate immediately several signaling pathways and transcription factors, thus regulating remodeling and differentiation. The immediate reaction of PDL fibroblasts to cyclic or static stress was tested so far in different experimental setups. Aim of this study was to compare the mechanotransduction caused by these two types of mechanical forces in human PDL fibroblasts. Time-course experiments revealed that both types of stress activate through phosphorylation all three members of mitogen-activated kinases (MAPK), with cyclic stress exhibiting a slightly more extended activation over static stress. Both stress modes upregulate the immediate-early genes C-FOS and C-JUN, encoding components of the activator protein-1 (AP-1). However, cells undergoing cyclic stress presented a more intense expression of both genes. These data indicate similar immediate/early responses of PDL fibroblasts to cyclic and static stress on the level of all three members of mitogen-activated kinases (MAPK). Furthermore, cyclic stress seems to have a significant higher impact on the expression levels of both C-FOS and C-JUN genes when compared to static stretch.
Main subject category:
Health Sciences
Keywords:
PDL, fibroblasts, tooth movement
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
35
Number of pages:
33

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