Τομέας Χειρουργικής
Library of the School of Health Sciences

2016-12-13

2016

Moustaki Margarita

Όθωνας Παπαδόπουλος, Καθηγητής,Ιατρική,ΕΚΠΑ
Αλκιβιάδης Κωστάκης,Καθηγητής,Ιατρική,ΕΚΠΑ
Δέσποινα Περρέα, Καθηγήτρια,Ιατρική,ΕΚΠΑ
Κωνσταντίνος Κόντζογλου,Αναπληρωτής Καθηγητής, Ιατρική,ΕΚΠΑ
Δημήτριος Δημητρούλης,Επίκουρος Καθηγητής,Ιατρική, ΕΚΠΑ
Χρίστος Βερύκοκος,Επίκουρος Καθηγητής,Ιατρική, ΕΚΠΑ
Μαρία Ρουμπελάκη,Επίκουρη Καθηγήτρια, Ιατρική,ΕΚΠΑ

Μελέτη παραγόντων βελτιστοποίησης των τεχνικών μεταφοράς και διατήρησης του αυτόλογου λίπους (fat transfer)

Greek

In vivo (animal) study on factors optimizing fat graft survival in autologous fat transfer

Backround: Traditional fat transplantation of fat grafting is a well- established technique and one of the standard approaches to soft-tissue reconstruction. The isolation of stem cells from adipose tissue inevitably led research to focus on the study of the combined transplantation of autologous fat and adipose derived stem cells (cell assisted lipotransfer-CAL).

Aim: This is an in vivo experimental study on cell-assisted lipotransfer with adipose derived stem cells on an animal model.The aim of this study is to prove and quantify the potential of adipose-derived stem cells to improve quality and long-term retention of fat grafts.

Methods: For the purposes of this study stem cells were isolated from C57BL/6J -GFP transgenic mice.Green Fluorescent Protein-positive stem cells were mixed with minced inguinal adipose tissue harvested from C57BL / 6JolaHsd mice and then co-implanted into Hsd: Athymic Nude-Foxn1nu mice (15). All mice had one side of their back grafted with the mixture and the contralateral control side grafted with the same amount of inguinal adipose tissue isolated from C57BL / 6JolaHsd mice. The survival of implanted Green-Fluorescent Protein-positive stem cells was tracked by in vivo fluorescence imaging for 56 days.

For the comparative study of the fat grafting survival the fat grafts were harvested from each mouse, weighed and subjected to histologic examination and immunohistochemistry staining of CD34 and Ki67 at 7 and 56 days.

Results: Results showed higher survival rate of the ADSC-enriched fat grafts (63% vs 33% ,p<0.05). In vivo fluorescence imaging proved that 8 weeks later, ADSCs are still present at the recipient area. Microvascular density of the ADSC-enriched grafts compared to the control grafts was significantly higher (p<0.05).

Conclusions: ADSC-enrichment is a promising optimization technique of autologous fat grafting resulting to a significant increase of the survival rate and micro-vascular density of fat grafts.

Fat transfer,Transplantation, Cell-assisted, Mesenchymal,Stem Cells,Stromal Cells,Adipose-derived stem cells, ADSC, Survival

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https://pergamos.lib.uoa.gr/uoa/dl/object/1326036