Μεταβολισμός και άσκηση

Doctoral Dissertation uoadl:1326546 383 Read counter

Unit:
Τομέας Παθολογίας
Library of the School of Health Sciences
Deposit date:
2017-01-09
Year:
2017
Author:
Sakelliou Alexandra
Dissertation committee:
Ασημίνα Μητράκου-Φαναριώτου, Αναπληρώτρια Καθηγήτρια, Ιατρική Σχολή, Ε.Κ.Π.Α.
Μαρία Αλεβιζάκη, Καθηγήτρια, Ιατρική Σχολή, Ε.Κ.Π.Α.
Ευάγγελος Τέρπος, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, Ε.Κ.Π.Α.
Μελπομένη Πέππα, Επίκουρη Καθηγήτρια, Ιατρική Σχολή, Ε.Κ.Π.Α.
Κίμωνας Σταματελόπουλος, Επίκουρος Καθηγητής, Ιατρική Σχολή, Ε.Κ.Π.Α.
Ειρήνη Λαμπρινουδάκη, Αναπληρώτρια Καθηγήτρια, Ιατρική Σχολή, Ε.Κ.Π.Α.
Ιωάννης Φατούρος, Αναπληρωτής Καθηγητής, Τμήμα ΤΕΦΑΑ, Πανεπιστήμιο Θεσσαλίας
Original Title:
Μεταβολισμός και άσκηση
Languages:
Greek
Summary:
We used thiol-based antioxidant supplementation (n-acetylcysteine, NAC) to determine whether immune mobilisation following skeletal muscle micro-trauma induced by exercise is redox-sensitive in healthy humans. According to a two-trial, double-blind, crossover, repeated measures design, 10 young men received either placebo or NAC (20 mg/kg/day) immediately after a muscle-damaging exercise protocol (300 eccentric contractions) and for eight consecutive days. Blood sampling and performance assessments were performed pre-exercise, post-exercise and daily throughout recovery. NAC reduced the decline of reduced glutathione in erythrocytes and the increase of plasma protein carbonyls, serum TAC and erythrocyte oxidized glutathione, TBARS and catalase activity during recovery thereby altering post-exercise redox status. The rise of muscle damage and inflammatory markers (muscle strength, creatine kinase activity, CRP, pro-inflammatory cytokines, adhesion molecules) was less pronounced in NAC during the first phase of recovery. The rise of leukocyte and neutrophil count was decreased by NAC post-exercise. Results on immune cell sub-populations obtained by flow cytometry indicated that NAC ingestion reduced the exercise-induced rise of total macrophages, HLA+ macrophages, and 11B+ macrophages and abolished the exercise-induced up-regulation of B lymphocytes. Natural killer cells declined only in PLA immediately post-exercise. These results indicate that thiol-based antioxidant supplementation blunts immune cell mobilisation in response to exercise-induced inflammation suggesting that leukocyte mobilization may be under redoxdependent regulation.
Keywords:
Exercise, Inflammation, Immune System, N-acetylcysteine, Reactive Oxygen Species
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
233
Number of pages:
133
ΕΘΝΙΚΟ ΚΑΙ ΚΑΠΟΔΙΣΤΡΙΑΚΟ ΠΑΝΕΠΙΣΤΗΜΙΟ ΑΘΗΝΩΝ.pdf (2 MB) Open in new window